Leslie A Sombers

Leslie A Sombers, Ph.D., M.A.

Professor

Department: Pharmacodynamics
Business Phone: (352) 273-7700
Business Email: leslie.sombers@ufl.edu

About Leslie A Sombers

Leslie Sombers is a Professor of Pharmacodynamics in the University of Florida College of Pharmacy. She earned her Ph.D. in Analytical Chemistry at Pennsylvania State University in 2008 under the direction of Andy Ewing, after which she researched as a postdoctoral fellow at The University of North Carolina at Chapel Hill in the R. Mark Wightman group. She spent 16 years as faculty at North Carolina State University where she built an interdisciplinary program comprising researchers from Chemistry, Biochemistry, Engineering, Biology and Physics. The Sombers lab research focuses on the development, characterization, optimization, and application of analytical techniques to study real-time neurochemical changes in preparations that range from single cells in culture to living brain tissue. She is the President-Elect of the Society for Electroanalytical Chemistry, she serves on the Board of Directors of the International Society for Monitoring Molecules in Neuroscience, and she also currently serves as an Associate Editor at ACS Chemical Neuroscience. Her research interests are focused on bioanalytical electrochemistry, biosensors, and measurement science.

Teaching Profile

Courses Taught
2024-2025
GMS6029 Brain Journal Club
2024-2025
GMS7794 Neuroscience Seminar
2024-2025
PHA7979 Advanced Research
2025
PHA6910 Supervised Research

Publications

Academic Articles
2024
Dioxythiophene/Nafion Polymer Composite Membranes for Tunable Size-Based Selectivity in the Voltammetric Detection of Small Neuropeptides.
ACS sensors. 9(10):5109-5115 [DOI] 10.1021/acssensors.4c00848. [PMID] 39319559.
2024
Electrochemical Quantification of Enkephalin Peptides Using Fast-Scan Cyclic Voltammetry.
Analytical chemistry. [DOI] 10.1021/acs.analchem.4c02418. [PMID] 39138126.
2024
Maximizing Electrochemical Information: A Perspective on Background-Inclusive Fast Voltammetry.
Analytical chemistry. 96(16):6097-6105 [DOI] 10.1021/acs.analchem.3c04938. [PMID] 38597398.
2024
Optimized Fabrication of Carbon-Fiber Microbiosensors for Codetection of Glucose and Dopamine in Brain Tissue.
ACS sensors. 9(5):2662-2672 [DOI] 10.1021/acssensors.4c00527. [PMID] 38689483.
2023
A Student Perspective on the 18th Monitoring Molecules in Neuroscience Meeting in Lyon.
ACS chemical neuroscience. 14(12):2253-2255 [DOI] 10.1021/acschemneuro.3c00271. [PMID] 37276431.
2023
Exploiting Microelectrode Geometry for Comprehensive Detection of Individual Exocytosis Events at Single Cells.
ACS sensors. 8(8):3187-3194 [DOI] 10.1021/acssensors.3c00884. [PMID] 37552870.
2023
Exploring Electrochemistry: A Hydrogen Peroxide Sensor Based on a Screen-Printed Carbon Electrode Modified with Prussian Blue.
Journal of chemical education. 100(12):4853-4859 [DOI] 10.1021/acs.jchemed.3c00844. [PMID] 38106547.
2023
Monitoring Molecules in Neuroscience.
ACS chemical neuroscience. 14(20):3726-3727 [DOI] 10.1021/acschemneuro.3c00592. [PMID] 37849295.
2023
Simultaneous, Real-Time Detection of Glutamate and Dopamine in Rat Striatum Using Fast-Scan Cyclic Voltammetry.
ACS sensors. 8(11):4091-4100 [DOI] 10.1021/acssensors.3c01267. [PMID] 37962541.
2023
Untapped Potential: Real-Time Measurements of Opioid Exocytosis at Single Cells.
Journal of the American Chemical Society. 145(44):24071-24080 [DOI] 10.1021/jacs.3c07487. [PMID] 37857375.
2022
Neurotransmitter Readily Escapes Detection at the Opposing Microelectrode Surface in Typical Amperometric Measurements of Exocytosis at Single Cells.
Analytical chemistry. 94(27):9548-9556 [DOI] 10.1021/acs.analchem.2c00060. [PMID] 35750055.
2022
Simultaneous Measurement of Striatal Dopamine and Hydrogen Peroxide Transients Associated with L-DOPA Induced Rotation in Hemiparkinsonian Rats.
ACS measurement science au. 2(2):120-131 [DOI] 10.1021/acsmeasuresciau.1c00030. [PMID] 36785724.
2022
Virtual Issue: Neurotoxicology.
ACS chemical neuroscience. 13(15):2238-2239 [DOI] 10.1021/acschemneuro.2c00375. [PMID] 35919966.
2020
Carbon-Fiber Nanoelectrodes for Real-Time Discrimination of Vesicle Cargo in the Native Cellular Environment.
ACS nano. 14(3):2917-2926 [DOI] 10.1021/acsnano.9b07318. [PMID] 32058693.
2020
Interpreting Dynamic Interfacial Changes at Carbon Fiber Microelectrodes Using Electrochemical Impedance Spectroscopy.
Langmuir : the ACS journal of surfaces and colloids. 36(15):4214-4223 [DOI] 10.1021/acs.langmuir.9b03941. [PMID] 32216254.
2020
Simultaneous voltammetric detection of glucose and lactate fluctuations in rat striatum evoked by electrical stimulation of the midbrain.
Analytical and bioanalytical chemistry. 412(24):6611-6624 [DOI] 10.1007/s00216-020-02797-0. [PMID] 32666141.
2019
Characterization of a Multiple-Scan-Rate Voltammetric Waveform for Real-Time Detection of Met-Enkephalin.
ACS chemical neuroscience. 10(4):2022-2032 [DOI] 10.1021/acschemneuro.8b00351. [PMID] 30571911.
2019
Drift Subtraction for Fast-Scan Cyclic Voltammetry Using Double-Waveform Partial-Least-Squares Regression.
Analytical chemistry. 91(11):7319-7327 [DOI] 10.1021/acs.analchem.9b01083. [PMID] 31081629.
2019
Local μ-Opioid Receptor Antagonism Blunts Evoked Phasic Dopamine Release in the Nucleus Accumbens of Rats.
ACS chemical neuroscience. 10(4):1935-1940 [DOI] 10.1021/acschemneuro.8b00437. [PMID] 30388365.
2019
NMDA Receptor-Dependent Cholinergic Modulation of Mesolimbic Dopamine Cell Bodies: Neurochemical and Behavioral Studies.
ACS chemical neuroscience. 10(3):1497-1505 [DOI] 10.1021/acschemneuro.8b00492. [PMID] 30412381.
2018
Carbon-Fiber Microbiosensor for Monitoring Rapid Lactate Fluctuations in Brain Tissue Using Fast-Scan Cyclic Voltammetry.
Analytical chemistry. 90(21):12994-12999 [DOI] 10.1021/acs.analchem.8b03694. [PMID] 30295022.
2018
Electrochemical Selectivity Achieved Using a Double Voltammetric Waveform and Partial Least Squares Regression: Differentiating Endogenous Hydrogen Peroxide Fluctuations from Shifts in pH.
Analytical chemistry. 90(3):1767-1776 [DOI] 10.1021/acs.analchem.7b03717. [PMID] 29243477.
2018
Fast-Scan Cyclic Voltammetry: Chemical Sensing in the Brain and Beyond.
Analytical chemistry. 90(1):490-504 [DOI] 10.1021/acs.analchem.7b04732. [PMID] 29182309.
2018
Quantitative Comparison of Enzyme Immobilization Strategies for Glucose Biosensing in Real-Time Using Fast-Scan Cyclic Voltammetry Coupled with Carbon-Fiber Microelectrodes.
Chemphyschem : a European journal of chemical physics and physical chemistry. 19(10):1197-1204 [DOI] 10.1002/cphc.201701235. [PMID] 29316144.
2018
Selective and Mechanically Robust Sensors for Electrochemical Measurements of Real-Time Hydrogen Peroxide Dynamics in Vivo.
Analytical chemistry. 90(1):888-895 [DOI] 10.1021/acs.analchem.7b03770. [PMID] 29191006.
2017
Background Signal as an in Situ Predictor of Dopamine Oxidation Potential: Improving Interpretation of Fast-Scan Cyclic Voltammetry Data.
ACS chemical neuroscience. 8(2):411-419 [DOI] 10.1021/acschemneuro.6b00325. [PMID] 28044445.
2017
Simultaneous Voltammetric Measurements of Glucose and Dopamine Demonstrate the Coupling of Glucose Availability with Increased Metabolic Demand in the Rat Striatum.
ACS chemical neuroscience. 8(2):272-280 [DOI] 10.1021/acschemneuro.6b00363. [PMID] 27984698.
2017
Spectroelectrochemical Characterization of the Dynamic Carbon-Fiber Surface in Response to Electrochemical Conditioning.
Langmuir : the ACS journal of surfaces and colloids. 33(32):7838-7846 [DOI] 10.1021/acs.langmuir.7b01443. [PMID] 28715197.
2016
The Hydroxyl Radical is a Critical Intermediate in the Voltammetric Detection of Hydrogen Peroxide.
Journal of the American Chemical Society. 138(8):2516-9 [DOI] 10.1021/jacs.5b13376. [PMID] 26840154.
2016
Unmasking the Effects of L-DOPA on Rapid Dopamine Signaling with an Improved Approach for Nafion Coating Carbon-Fiber Microelectrodes.
Analytical chemistry. 88(16):8129-36 [DOI] 10.1021/acs.analchem.6b01871. [PMID] 27441547.
2014
Multiple scan rate voltammetry for selective quantification of real-time enkephalin dynamics.
Analytical chemistry. 86(15):7806-12 [DOI] 10.1021/ac501725u. [PMID] 24967837.
2014
Peroxygenase and oxidase activities of dehaloperoxidase-hemoglobin from Amphitrite ornata.
Journal of the American Chemical Society. 136(22):7914-25 [DOI] 10.1021/ja500293c. [PMID] 24791647.
2013
Carbon nanotube yarn electrodes for enhanced detection of neurotransmitter dynamics in live brain tissue.
ACS nano. 7(9):7864-73 [DOI] 10.1021/nn402857u. [PMID] 23941323.
2013
Enzyme-modified carbon-fiber microelectrode for the quantification of dynamic fluctuations of nonelectroactive analytes using fast-scan cyclic voltammetry.
Analytical chemistry. 85(18):8780-6 [DOI] 10.1021/ac4017852. [PMID] 23919631.
2013
In situ electrode calibration strategy for voltammetric measurements in vivo.
Analytical chemistry. 85(23):11568-75 [DOI] 10.1021/ac402884n. [PMID] 24224460.
2013
Quantitation of hydrogen peroxide fluctuations and their modulation of dopamine dynamics in the rat dorsal striatum using fast-scan cyclic voltammetry.
ACS chemical neuroscience. 4(5):782-9 [DOI] 10.1021/cn4000499. [PMID] 23556461.
2013
Real-time chemical measurements of dopamine release in the brain.
Methods in molecular biology (Clifton, N.J.). 964:275-94 [DOI] 10.1007/978-1-62703-251-3_16. [PMID] 23296789.
2012
Sources contributing to the average extracellular concentration of dopamine in the nucleus accumbens.
Journal of neurochemistry. 121(2):252-62 [DOI] 10.1111/j.1471-4159.2012.07677.x. [PMID] 22296263.
2012
Trace metal complexation by the triscatecholate siderophore protochelin: structure and stability.
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine. 25(2):393-412 [DOI] 10.1007/s10534-011-9513-7. [PMID] 22187125.
2011
Comparison of electrode materials for the detection of rapid hydrogen peroxide fluctuations using background-subtracted fast scan cyclic voltammetry.
The Analyst. 136(17):3550-6 [DOI] 10.1039/c1an15337d. [PMID] 21727955.
2010
Specific oxygen-containing functional groups on the carbon surface underlie an enhanced sensitivity to dopamine at electrochemically pretreated carbon fiber microelectrodes.
Langmuir : the ACS journal of surfaces and colloids. 26(11):9116-22 [DOI] 10.1021/la9048924. [PMID] 20166750.
2010
Voltammetric detection of hydrogen peroxide at carbon fiber microelectrodes.
Analytical chemistry. 82(12):5205-10 [DOI] 10.1021/ac100536s. [PMID] 20503997.
2009
Synaptic overflow of dopamine in the nucleus accumbens arises from neuronal activity in the ventral tegmental area.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 29(6):1735-42 [DOI] 10.1523/JNEUROSCI.5562-08.2009. [PMID] 19211880.
2008
Dopamine detection with fast-scan cyclic voltammetry used with analog background subtraction.
Analytical chemistry. 80(11):4040-8 [DOI] 10.1021/ac800108j. [PMID] 18433146.
2007
Dopamine release is heterogeneous within microenvironments of the rat nucleus accumbens.
The European journal of neuroscience. 26(7):2046-54 [PMID] 17868375.
2007
High osmolarity and L-DOPA augment release via the fusion pore in PC12 cells.
Chemphyschem : a European journal of chemical physics and physical chemistry. 8(17):2471-7 [PMID] 17966970.
2007
Multicore vesicles: hyperosmolarity and L-DOPA induce homotypic fusion of dense core vesicles.
Cellular and molecular neurobiology. 27(5):681-5 [PMID] 17554620.
2007
Phasic dopamine release evoked by abused substances requires cannabinoid receptor activation.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 27(4):791-5 [PMID] 17251418.
2005
Correlation between vesicle quantal size and fusion pore release in chromaffin cell exocytosis.
Biophysical journal. 88(6):4411-20 [PMID] 15792983.
2005
Loaded dopamine is preferentially stored in the halo portion of PC12 cell dense core vesicles.
Journal of neurochemistry. 93(5):1122-31 [PMID] 15934933.
2004
The effects of vesicular volume on secretion through the fusion pore in exocytotic release from PC12 cells.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 24(2):303-9 [PMID] 14724228.
2003
Artificial cells: unique insights into exocytosis using liposomes and lipid nanotubes.
Proceedings of the National Academy of Sciences of the United States of America. 100(2):400-4 [PMID] 12514323.
2002
Differentiated PC12 cells: a better model system for the study of the VMAT’s effects on neuronal communication.
Annals of the New York Academy of Sciences. 971:86-8 [PMID] 12438095.

Grants

Sep 2024 ACTIVE
Expanding the Scope and Usability of Fast Voltammetric Measurements
Role: Principal Investigator
Funding: NATL INST OF HLTH NIDA

Contact Details

Phones:
Business:
(352) 273-7700
Emails:
Addresses:
Business Street:
PO Box 100487; rm P1-20
1345 CENTER DR
GAINESVILLE FL 32610