Microbial Genomics and Biosynthetic Engineering
Genomic screening of marine cyanobacteria for the detection of targeted secondary metabolite biosynthetic genes has been achieved through fosmid DNA libraries construction using HMW metagenomic DNA isolated from environmental cyanobacterial samples. Conserved enzymatic steps shared by diverse biosynthetic gene clusters are used as starting point for the design of degenerate primers from the corresponding biosynthetic...