Sihong Song

Sihong Song, Ph.D.

Associate Professor

Department: Pharmaceutics
Business Phone: (352) 273-7867
Business Email: shsong@ufl.edu

Research Profile

Song’s interests are in 1) improvement of safety and efficiency of rAAV vector by understanding molecular mechanisms of persistence and integration of the AAV genome in mammalian cells and 2) the use of recombinant Adeno-Associated Virus (rAAV) vectors mediated gene transfer to develop gene therapy approaches for common diseases such as diabetes and arthritis.

His research interests include: molecular and cell biology, gene expression and gene regulation, liver and muscle gene delivery, gene therapy for type 1 diabetes and alpha 1 antitrypsin deficiency, adeno-associated virus (AAV) vector biology: DNA persistence, integration and interaction with cellular factors; host immune responses to transgene products

Publications

2019
Alpha-1-Antitrypsin Ameliorates Pristane Induced Diffuse Alveolar Hemorrhage in Mice.
Journal of clinical medicine. 8(9) [DOI] 10.3390/jcm8091341. [PMID] 31470606.
2019
Regulated hAAT Expression from a Novel rAAV Vector and Its Application in the Prevention of Type 1 Diabetes.
Journal of clinical medicine. 8(9) [DOI] 10.3390/jcm8091321. [PMID] 31466263.
2018
Alpha 1 Antitrypsin Gene Therapy Extends the Lifespan of Lupus-Prone Mice.
Molecular therapy. Methods & clinical development. 11:131-142 [DOI] 10.1016/j.omtm.2018.10.007. [PMID] 30547047.
2018
Alpha-1 Antitrypsin Therapy for Autoimmune Disorders.
Chronic obstructive pulmonary diseases (Miami, Fla.). 5(4):289-301 [DOI] 10.15326/jcopdf.5.4.2018.0131. [PMID] 30723786.
2018
Anti-inflammaging effects of human alpha-1 antitrypsin.
Aging cell. 17(1) [DOI] 10.1111/acel.12694. [PMID] 29045001.
2018
Gene Delivery of Alpha-1-Antitrypsin Using Recombinant Adeno-Associated Virus (rAAV).
Methods in molecular biology (Clifton, N.J.). 1826:183-196 [DOI] 10.1007/978-1-4939-8645-3_12. [PMID] 30194601.
2018
In Vivo Analysis of Alpha-1-Antitrypsin Functions in Autoimmune Disease Models.
Methods in molecular biology (Clifton, N.J.). 1826:143-155 [DOI] 10.1007/978-1-4939-8645-3_10. [PMID] 30194599.
2017
Alpha-1 antitrypsin inhibits RANKL-induced osteoclast formation and functions.
Molecular medicine (Cambridge, Mass.). 23:57-69 [DOI] 10.2119/molmed.2016.00170. [PMID] 28332697.
2017
Electroacupuncture Relieves Depression-Like Symptoms in Rats Exposed to Chronic Unpredictable Mild Stress By Activating Erk Signaling Pathway
Neuroscience Letters. 642:43-50 [DOI] 10.1016/j.neulet.2017.01.060.
2017
Ischemia-responsive protein 94 is a key mediator of ischemic neuronal injury-induced microglial activation.
Journal of neurochemistry. 142(6):908-919 [DOI] 10.1111/jnc.14111. [PMID] 28640931.
2017
Transplantation of Adipose Tissue-Derived Mesenchymal Stem Cell (ATMSC) Expressing Alpha-1 Antitrypsin Reduces Bone Loss in Ovariectomized Osteoporosis Mice.
Human gene therapy. 28(2):179-189 [DOI] 10.1089/hum.2016.069. [PMID] 27802778.
2016
Alpha 1 Antitrypsin Inhibits Dendritic Cell Activation and Attenuates Nephritis in a Mouse Model of Lupus.
PloS one. 11(5) [DOI] 10.1371/journal.pone.0156583. [PMID] 27232337.
2016
Alpha-1 Antitrypsin Gene Therapy Ameliorates Bone Loss in Ovariectomy-Induced Osteoporosis Mouse Model.
Human gene therapy. 27(9):679-86 [DOI] 10.1089/hum.2016.029. [PMID] 27158796.
2015
In Situ Transplantation of Alginate Bioencapsulated Adipose Tissues Derived Stem Cells (ADSCs) via Hepatic Injection in a Mouse Model.
PloS one. 10(9) [DOI] 10.1371/journal.pone.0138184. [PMID] 26372641.
2014
Alpha 1-antitrypsin therapy mitigated ischemic stroke damage in rats.
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association. 23(5):e355-63 [DOI] 10.1016/j.jstrokecerebrovasdis.2013.12.029. [PMID] 24582784.
2014
Reprogramming adipose tissue-derived mesenchymal stem cells into pluripotent stem cells by a mutant adeno-associated viral vector.
Human gene therapy methods. 25(1):72-82 [DOI] 10.1089/hgtb.2013.011. [PMID] 24191859.
2013
Retinoid X receptor agonists modulate Foxp3⁺ regulatory T cell and Th17 cell differentiation with differential dependence on retinoic acid receptor activation.
Journal of immunology (Baltimore, Md. : 1950). 191(7):3725-33 [DOI] 10.4049/jimmunol.1300032. [PMID] 23980207.
2012
Alpha-1 Antitrypsin Reduces Ovariectomy-Induced Bone Loss in Mice
The FASEB's Journal. 26
2011
Adipose tissue-derived mesenchymal stem cell-based liver gene delivery.
Journal of hepatology. 54(5):930-8 [DOI] 10.1016/j.jhep.2010.07.051. [PMID] 21168381.
2011
Alpha-1 antitrypsin protein and gene therapies decrease autoimmunity and delay arthritis development in mouse model.
Journal of translational medicine. 9 [DOI] 10.1186/1479-5876-9-21. [PMID] 21345239.
2011
Alpha-1 antitrypsin reduces ovariectomy-induced bone loss in mice.
Annals of the New York Academy of Sciences. 1240:E31-5 [DOI] 10.1111/j.1749-6632.2011.06370.x. [PMID] 22360827.
2011
The protective effects of plasma gelsolin on stroke outcome in rats.
Experimental & translational stroke medicine. 3(1) [DOI] 10.1186/2040-7378-3-13. [PMID] 22047744.
2010
Combination of alpha-1 antitrypsin and doxycycline suppresses collagen-induced arthritis.
The journal of gene medicine. 12(1):35-44 [DOI] 10.1002/jgm.1409. [PMID] 19866480.
2010
Efficient induction of CCR9 on T cells requires coactivation of retinoic acid receptors and retinoid X receptors (RXRs): exaggerated T Cell homing to the intestine by RXR activation with organotins.
Journal of immunology (Baltimore, Md. : 1950). 185(9):5289-99 [DOI] 10.4049/jimmunol.1000101. [PMID] 20881191.
2010
Ex vivo transduction and transplantation of bone marrow cells for liver gene delivery of alpha1-antitrypsin.
Molecular therapy : the journal of the American Society of Gene Therapy. 18(8):1553-8 [DOI] 10.1038/mt.2010.116. [PMID] 20551917.
2010
Feasibility of localized immunosuppression: 2. PLA microspheres for the sustained local delivery of a soft immunosuppressant.
Die Pharmazie. 65(6):429-35 [PMID] 20614691.
View on: PubMed
2010
Intradermal alpha1-antitrypsin therapy avoids fatal anaphylaxis, prevents type 1 diabetes and reverses hyperglycaemia in the NOD mouse model of the disease.
Diabetologia. 53(10):2198-204 [DOI] 10.1007/s00125-010-1829-2. [PMID] 20593162.
2010
The effect of DNA-dependent protein kinase on adeno-associated virus replication.
PloS one. 5(12) [DOI] 10.1371/journal.pone.0015073. [PMID] 21188139.
2009
Distinct immune responses to transgene products from rAAV1 and rAAV8 vectors.
Proceedings of the National Academy of Sciences of the United States of America. 106(40):17158-62 [DOI] 10.1073/pnas.0909520106. [PMID] 19805176.
2009
Immune depletion with cellular mobilization imparts immunoregulation and reverses autoimmune diabetes in nonobese diabetic mice.
Diabetes. 58(10):2277-84 [DOI] 10.2337/db09-0557. [PMID] 19628781.
2008
Alpha-1 antitrypsin treatment of spontaneously diabetic nonobese diabetic mice receiving islet allografts.
Transplantation proceedings. 40(2):457-8 [DOI] 10.1016/j.transproceed.2008.02.010. [PMID] 18374100.
2008
Human alpha 1-antitrypsin therapy induces fatal anaphylaxis in non-obese diabetic mice.
Clinical and experimental immunology. 154(1):15-21 [DOI] 10.1111/j.1365-2249.2008.03721.x. [PMID] 18759852.
2008
Prolonged islet allograft survival by alpha-1 antitrypsin: the role of humoral immunity.
Transplantation proceedings. 40(2):455-6 [DOI] 10.1016/j.transproceed.2008.02.009. [PMID] 18374099.
2007
Alpha1-antitrypsin protects beta-cells from apoptosis.
Diabetes. 56(5):1316-23 [PMID] 17360983.
View on: PubMed
2007
Development of immunocompetent lymphocytes in vivo from murine umbilical cord blood cells.
Transplantation. 84(1):23-30 [PMID] 17627233.
View on: PubMed
2006
A novel antiapoptotic role for alpha1-antitrypsin in the prevention of pulmonary emphysema.
American journal of respiratory and critical care medicine. 173(11):1222-8 [PMID] 16514110.
View on: PubMed
2006
Alpha1-antitrypsin gene therapy modulates cellular immunity and efficiently prevents type 1 diabetes in nonobese diabetic mice.
Human gene therapy. 17(6):625-34 [PMID] 16776571.
View on: PubMed
2006
Phase I trial of intramuscular injection of a recombinant adeno-associated virus serotype 2 alphal-antitrypsin (AAT) vector in AAT-deficient adults.
Human gene therapy. 17(12):1177-86 [PMID] 17115945.
View on: PubMed
2006
Therapeutic level of functional human alpha 1 antitrypsin (hAAT) secreted from murine muscle transduced by adeno-associated virus (rAAV1) vector.
The journal of gene medicine. 8(6):730-5 [PMID] 16518879.
View on: PubMed
2005
Efficient hepatic delivery and expression from a recombinant adeno-associated virus 8 pseudotyped alpha1-antitrypsin vector.
Molecular therapy : the journal of the American Society of Gene Therapy. 12(5):867-75 [PMID] 16085464.
View on: PubMed
2005
Enhancing rAAV vector expression in the lung.
The journal of gene medicine. 7(7):842-50 [PMID] 15838934.
View on: PubMed
2005
Glucose transporter-2 (GLUT2) promoter mediated transgenic insulin production reduces hyperglycemia in diabetic mice.
FEBS letters. 579(25):5759-64 [PMID] 16223491.
View on: PubMed
2004
DNA-dependent PK inhibits adeno-associated virus DNA integration.
Proceedings of the National Academy of Sciences of the United States of America. 101(7):2112-6 [PMID] 14766968.
View on: PubMed
2004
Ex vivo transduced liver progenitor cells as a platform for gene therapy in mice.
Hepatology (Baltimore, Md.). 40(4):918-24 [PMID] 15382177.
View on: PubMed
2004
Retinoic acid imprints gut-homing specificity on T cells.
Immunity. 21(4):527-38 [PMID] 15485630.
View on: PubMed
2003
Full reconstitution of hematopoietic system by murine umbilical cord blood.
Transplantation. 75(11):1820-6 [PMID] 12811240.
View on: PubMed
2003
Successful cryopreservation of mouse ovaries by vitrification.
Biology of reproduction. 68(3):881-7 [PMID] 12604638.
View on: PubMed
2003
Systemic overexpression of IL-10 induces CD4+CD25+ cell populations in vivo and ameliorates type 1 diabetes in nonobese diabetic mice in a dose-dependent fashion.
Journal of immunology (Baltimore, Md. : 1950). 171(5):2270-8 [PMID] 12928371.
View on: PubMed
2002
Improved method of recombinant AAV2 delivery for systemic targeted gene therapy.
Molecular therapy : the journal of the American Society of Gene Therapy. 6(1):106-12 [PMID] 12095310.
View on: PubMed
2002
Intramuscular administration of recombinant adeno-associated virus 2 alpha-1 antitrypsin (rAAV-SERPINA1) vectors in a nonhuman primate model: safety and immunologic aspects.
Molecular therapy : the journal of the American Society of Gene Therapy. 6(3):329-35 [PMID] 12231168.
View on: PubMed

Grants

Jul 2020 ACTIVE
The circadian clock protein BMAL and post-translational regulation of ENaC in the kidney
Role: Co-Investigator
Funding: NATL INST OF HLTH NIDDK
Jun 2020 ACTIVE
Development of a novel gene therapy for lupus
Role: Principal Investigator
Funding: SAREPTA THERAPEUTICS
Mar 2018 – Nov 2018
Testing of AAV-hAAT Vectors in Mice
Role: Principal Investigator
Funding: APPLIED GENETICS TECH CORP
Aug 2016 – Aug 2018
Study on the inter and intra generic transmission of drug resistance determinants
Role: Principal Investigator
Funding: EGYPTIAN CULTURAL AND EDU BUR
Jun 2015 – May 2018
60919270Alpha 1 antitrypsin for the treatment of osteoporosis
Role: Principal Investigator
Funding: UF DSR OPPORTUNITY FUND
Jan 2015 – Jan 2017
The Protective Effect of Prolostin C for the Treatment of Lupus
Role: Principal Investigator
Funding: GRIFOLS WORLDWIDE OPERATIONS LTD

Patents

Published June 2008
rAAV Vector-Based Compositions and Methods for the Prevention and Treatment of Mammalian Diseases
#US 2009/0111766 A1
Published October 2007
Adeno-Associated Viral Vectors for the Treatment and Prevention of Diabetes (CON)
#US-2009-0186002-A1
Published August 2005
rAAV Vector-Based Compositions and methods for the Prevention and Treatment of Mammalian Diseases
#US2007/0003518A1
Published January 2003
Adeno-Associated Viral Vectors for the Treatment and Prevention of Diabetes
#US-2003-0095949-A1
Published October 2002
rAAV Compositions for Gene Therapy
#US-2003-0082162-A1
Issued October 2002
Materials and Methods for Gene Therapy
#6,461,606

Education

Post Doctoral Associate in Gene Therapy
1999 · University of Florida
Ph.D.
1996 · University of Florida
M.S.
1989 · Jilin Agricultural University
B.S.
1982 · Jilin Agricultural University

Teaching Profile

Courses Taught
2013-2021
PHA5172 Biotech and Drug Devlp
2016,2019-2021
PHA6910 Supervised Research
2016,2020-2021
GMS6251 Molecular Therapy I ? Vectors and Molecular Mechanisms
2014-2021
PHA6938 Research Seminar
2008-2021
PHA7979 Advanced Research
2008-2021
PHA7980 Research for Doctoral Dissertation
2010-2014,2020-2021
PHA6971 Research for Master’s Thesis
2007,2009-2011,2011-2015,2013-2019,2015-2021,2017-2021,2019-2021
PHA6183 Pharmaceutical Gene Delivery
2019-2021
PHA7939 Journal Colloquy in Pharmacodynamics
2014,2016
PHA5905 Clin Pharm Research
2016
PHA6894 Introduction to Graduate Studies
2009-2014
PHA5451 Clinical Biochemistry
2011
PHA6935 Sel Topics Pharmacy

Contact Details

Phones:
Business:
(352) 273-7867
Emails:
Business:
shsong@ufl.edu