Jason M Puglise

Jason M Puglise,

Biological Scientist II

Department: Pharmacotherapy & Translational Research
Business Phone: (352) 273-7858
Business Email: jpuglise@ufl.edu

About Jason M Puglise

Military veteran with multi-faceted skill sets with diverse research background. Served as a course instructor in both military and college environments and worked for government, biotech company, and presently academia. Been a Biological Scientist at UF for several years.

Clinical Profile

From 1992-1996, I served in the U.S. Army as a field combat medic (Medical Specialist, 91B10), stationed overseas as well as in the continental United States. I was introduced to the medical field through lectures, demonstrations, practical exercises, and field trainings which covered topics such as anatomy and physiology, CPR, and first aid. I learned medical terminology via assisting with the medical care and examination of patients (aka performed sick call), by maintaining health and clinical files, and by performing duties related to emergency treatment. I received EMT training, worked in a hospital setting at Ft. Bragg, and have interacted with nurses, doctors, and other medical personnel from various departments. Stationed in Germany, I taught a Combat Lifesaving course to my fellow soldiers for two years instructing them on topics such as first aid, I.V. therapy, and triage. Additionally, I participated in notable events such as Belgium’s Commemoration of the 50th Anniversary of their liberation by the Allies (1994) and the International Nijmegen Marches (1993). While attached to 28th CSH Bravo Co., we provided medical support to the US Virgin Islands during Hurricane Marilyn Federal Relief Operations (1995).

Research Profile

As a graduate student, my master’s research involved genetic/genomic work in the nematode genus Caenorhabditis. As a visiting doctoral student at the University of Chicago, I focused on epithelial biology, cytoskeletal dynamics, and the protein kinase Pak1. I primarily focused on the role that Pak1 plays in certain biological processes in renal epithelial cells. Specifically, my dissertation work examined the role that Pak1 and its binding partner PIX play in cytokinesis, epithelial morphogenesis, and ciliogenesis in Madin-Darby canine kidney (MDCK) cells.

I’ve held several science positions. For about two years, I worked for USDA as a molecular biologist/support scientist and conducted research focusing on mosquito control via RNAi delivery. I examined the environmental stability of dsRNA molecules and determined whether inhibitors of apoptosis (IAPs) would be suitable RNAi targets in Aedes, Anopheles, and Culex mosquitoes. At the University of Florida, in June of 2016, I crossed over into the field of muscle biology, and became a member of the Physiological Assessment Core for the Wellstone/Myology Institute. As a Core member and Biological Scientist I-II (technician), I conducted ex vivo and in vivo muscle function testing on mice for numerous research projects within the Myology Institute community as well as outside collaborators. This entailed in vitro muscle mechanics on the soleus, extensor digitorum longus, and diaphragm, and includes in situ testing on the tibialis anterior. For roughly five years, I harvested tissues and performed non-survival surgeries related to muscle function testing on hundreds of mice of various strains each year. From May 2021 to March 2024 in the College of Medicine, I worked for the OB/GYN Department in the Center for Research in Perinatal Outcomes. As a Biological Scientist III/Lab Manager, I maintained and ensure general lab upkeep, functioned as a liaison between laboratory, administrative, and business partners, and provided technical assistance in support of placental and perinatal research. Currently, I work for the Dept. of Pharmacotherapy and Translational Research and am involved with breast cancer research.

Open Researcher and Contributor ID (ORCID)

0000-0002-5913-130X

Publications

2022
Isolated murine skeletal muscles utilize pyruvate over glucose for oxidation.
Metabolomics : Official journal of the Metabolomic Society. 18(12) [DOI] 10.1007/s11306-022-01948-x. [PMID] 36480060.
2022
Novel γ-sarcoglycan interactors in murine muscle membranes
Skeletal Muscle. 12(1) [DOI] 10.1186/s13395-021-00285-2.
2021
Antagonistic control of myofiber size and muscle protein quality control by the ubiquitin ligase UBR4 during aging.
Nature communications. 12(1) [DOI] 10.1038/s41467-021-21738-8. [PMID] 33658508.
2019
Deletion of muscle IGF-I transiently impairs growth and progressively disrupts glucose homeostasis in male mice.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 33(1):181-194 [DOI] 10.1096/fj.201800459R. [PMID] 29932867.
2016
Expression Profiles and RNAi Silencing of Inhibitor of Apoptosis Transcripts in Aedes, Anopheles, and Culex Mosquitoes (Diptera: Culicidae).
Journal of medical entomology. 53(2):304-14 [PMID] 26659858.
2012
Pak1 regulates the orientation of apical polarization and lumen formation by distinct pathways.
PloS one. 7(7) [DOI] 10.1371/journal.pone.0041039. [PMID] 22815903.
2010
Cadherins and Pak1 control contact inhibition of proliferation by Pak1-betaPIX-GIT complex-dependent regulation of cell-matrix signaling.
Molecular and cellular biology. 30(8):1971-83 [DOI] 10.1128/MCB.01247-09. [PMID] 20154149.
2010
Roles of the Rac/Cdc42 effector proteins Pak and PIX in cytokinesis, ciliogenesis, and cyst formation in renal epithelial cells
. [DOI] http://rave.ohiolink.edu/etdc/view?acc_num=ucin1288980975.

Education

PhD in Cell & Molecular Biology
2005-2010 · University of Cincinnati; Cincinnati, OH
MS in Biological Sciences
2001-2005 · Wright State University; Dayton, OH
BA & BS in Biological Sciences
1997-2001 · Wright State University; Dayton, OH
Military Education (College credit earned)
1992-1996 · AARTS Operation Center; Ft. Leavenworth, KS (Military Education)
N/A (College credit earned)
1991-1992 · Ohio State University- Branch Campus; Lima, OH (Ohio State Academy Program Option B, Senate Bill 140, Post-Secondary Option)

Contact Details

Phones:
Business:
(352) 273-7858
Emails:
Business:
jpuglise@ufl.edu
Addresses:
Business Mailing:
PO Box 100486
GAINESVILLE FL 32610
Business Street:
1225 Center Drive
GAINESVILLE FL 32610