Eric Krause

Eric Krause, Ph.D.

Associate Professor And Director Of The Center For Integrative Cardiovascular And Metabolic Disease

Department: Pharmacodynamics
Business Phone: (352) 273-6977
Business Email: ekrause@cop.ufl.edu

Research Profile

Stress, broadly defined as a real or perceived threat to homeostasis, activates neural circuits that alter the body’s physiology and behavior to ensure survival and well-being. The Krause Lab employs the rodent model to investigate how the central nervous system coordinates behavioral and physiological responses to stress. In particular, the lab studies how signals associated with dehydration impact the central pathways regulating stress responding and mood.

For example, loss of body fluids increases circulating levels of angiotensin II (ANGII), which activates the angiotensin type-1 receptors (AT1R) in various tissues to increase fluid intake, promote water and electrolyte retention and elevate blood pressure. Interestingly, the same neural circuits that regulate the influence of ANGII on hydromineral balance and cardiovascular function are also recruited during responding to psychological stress. Our studies have demonstrated that ANGII targets the brain via activation of AT1R in the subfornical organ (SFO), a specialized nucleus with an incomplete blood-brain-barrier, to coordinate the endocrine, cardiovascular and behavioral limbs of the stress response. These observations have led us to hypothesize that the AT1R in the SFO may be the site of convergence for the co-morbidity of psychopathology and cardiovascular disease.

In contrast to loss of body fluids, increasing plasma sodium concentration (pNa) elicits thirst by activating osmoreceptors in the brain. Acute increases in pNa suppress circulating ANGII and greatly elevate oxytocin levels centrally and systemically. Given that ANGII stimulates stress-responding and oxytocin is documented to have anti-stress properties especially when the stressor that is employed is social in nature, we predicted that acute osmotic dehydration would attenuate stress-responsiveness. Indeed, our studies have found that rats systemically administered a concentrated NaCl solution have decreased endocrine and cardiovascular responses to psychological stress exposure. Additionally, rats given NaCl engaged in more interactions when presented with unfamiliar conspecifics, suggesting that acute salt-loading decreases social anxiety. These observations have led to the hypothesis that acute increases in pNa attenuate stress-responsiveness, which may promote the social interactions that are encountered when engaging in drinking behavior. We are currently using this model to provide insight towards mechanisms underlying psychopathologies accompanied by cardiovascular irregularities like social phobias, anxiety and panic disorders.

My research utilizes an integrative approach towards investigating brain circuits governing physiology and behavior. We use chronic stress paradigms (i.e. visual burrow system or chronic variable stress) to model psychiatric disorders in rodents. Tract tracing, immunohistochemistry and in situ hybridization are used to characterize the neuroanatomical circuits mediating stress responding. Subsequently, we administer lentiviral vectors into brain nuclei that compose the circuit to inhibit the expression of targeted genes and reveal their contribution to stress responding and mood. To evaluate the limbs of the stress response, hormonal, cardiovascular and behavioral outcomes are assessed. Recently, we’ve obtained the angiotensin receptor flox mouse and plan to employ the cre-lox system to inhibit the expression of this receptor in select tissues.

Publications

2021
An Angiotensin-Responsive Connection from the Lamina Terminalis to the Paraventricular Nucleus of the Hypothalamus Evokes Vasopressin Secretion to Increase Blood Pressure in Mice.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 41(7):1429-1442 [DOI] 10.1523/JNEUROSCI.1600-20.2020. [PMID] 33328294.
2021
Central and peripheral GLP-1 systems independently suppress eating.
Nature metabolism. 3(2):258-273 [DOI] 10.1038/s42255-021-00344-4. [PMID] 33589843.
2021
Dendritic osmosensors modulate activity-induced calcium influx in oxytocinergic magnocellular neurons of the mouse PVN.
eLife. 10 [DOI] 10.7554/eLife.63486. [PMID] 34250900.
2021
Identification of Novel Cross-Talk between the Neuroendocrine and Autonomic Stress Axes Controlling Blood Pressure.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 41(21):4641-4657 [DOI] 10.1523/JNEUROSCI.0251-21.2021. [PMID] 33858944.
2021
Targeting angiotensin type 2 receptors located on pressor neurons in the nucleus of the solitary tract to relieve hypertension in mice.
Cardiovascular research. [DOI] 10.1093/cvr/cvab085. [PMID] 33723600.
2020
Brain Angiotensin Type-1 and Type-2 Receptors in Physiological and Hypertensive Conditions: Focus on Neuroinflammation.
Current hypertension reports. 22(7) [DOI] 10.1007/s11906-020-01062-0. [PMID] 32661792.
2020
Brain angiotensin type-1 and type-2 receptors: cellular locations under normal and hypertensive conditions.
Hypertension research : official journal of the Japanese Society of Hypertension. 43(4):281-295 [DOI] 10.1038/s41440-019-0374-8. [PMID] 31853042.
2020
Endogenous oxytocin inhibits hypothalamic corticotrophin-releasing hormone neurones following acute hypernatraemia.
Journal of neuroendocrinology. 32(3) [DOI] 10.1111/jne.12839. [PMID] 32133707.
2020
Experimentally Manipulated Low Social Status and Food Insecurity Alter Eating Behavior Among Adolescents: A Randomized Controlled Trial.
Obesity (Silver Spring, Md.). 28(11):2010-2019 [DOI] 10.1002/oby.23002. [PMID] 33150744.
2020
Overexpression of angiotensin converting enzyme 2 reduces anxiety-like behavior in female mice.
Physiology & behavior. 224 [DOI] 10.1016/j.physbeh.2020.113002. [PMID] 32525008.
2020
Oxytocin treatment for alcoholism: Potential neurocircuitry targets.
Neuropharmacology. 171 [DOI] 10.1016/j.neuropharm.2020.108091. [PMID] 32304701.
2019
An anti-CRF antibody suppresses the HPA axis and reverses stress-induced phenotypes.
The Journal of experimental medicine. 216(11):2479-2491 [DOI] 10.1084/jem.20190430. [PMID] 31467037.
2019
Angiotensin receptor expression revealed by reporter mice and beneficial effects of AT2R agonist in retinal cells.
Experimental eye research. 187 [DOI] 10.1016/j.exer.2019.107770. [PMID] 31449794.
2019
Oxytocin Receptors Are Expressed by Glutamatergic Prefrontal Cortical Neurons That Selectively Modulate Social Recognition.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 39(17):3249-3263 [DOI] 10.1523/JNEUROSCI.2944-18.2019. [PMID] 30804095.
2018
A novel rat model of comorbid PTSD and addiction reveals intersections between stress susceptibility and enhanced cocaine seeking with a role for mGlu5 receptors.
Translational psychiatry. 8(1) [DOI] 10.1038/s41398-018-0265-9. [PMID] 30291225.
2018
Angiotensin II Triggers Peripheral Macrophage-to-Sensory Neuron Redox Crosstalk to Elicit Pain.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 38(32):7032-7057 [DOI] 10.1523/JNEUROSCI.3542-17.2018. [PMID] 29976627.
2018
Coupling corticotropin-releasing-hormone and angiotensin converting enzyme 2 dampens stress responsiveness in male mice.
Neuropharmacology. 133:85-93 [DOI] 10.1016/j.neuropharm.2018.01.025. [PMID] 29360543.
2018
Macrophage angiotensin II type 2 receptor triggers neuropathic pain.
Proceedings of the National Academy of Sciences of the United States of America. 115(34):E8057-E8066 [DOI] 10.1073/pnas.1721815115. [PMID] 30082378.
2018
Stress-induced corticosterone secretion covaries with working memory in aging.
Neurobiology of aging. 71:156-160 [DOI] 10.1016/j.neurobiolaging.2018.07.015. [PMID] 30144648.
2017
A Single Angiotensin II Hypertensive Stimulus Is Associated with Prolonged Neuronal and Immune System Activation in Wistar-Kyoto Rats.
Frontiers in physiology. 8 [DOI] 10.3389/fphys.2017.00592. [PMID] 28912720.
2017
A Unique “Angiotensin-Sensitive” Neuronal Population Coordinates Neuroendocrine, Cardiovascular, and Behavioral Responses to Stress.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 37(13):3478-3490 [DOI] 10.1523/JNEUROSCI.3674-16.2017. [PMID] 28219987.
2017
Chronic salt-loading reduces basal excitatory input to CRH neurons in the paraventricular nucleus and accelerates recovery from restraint stress in male mice.
Physiology & behavior. 176:189-194 [DOI] 10.1016/j.physbeh.2017.03.038. [PMID] 28351560.
2017
Editorial.
Physiology & behavior. 178 [DOI] 10.1016/j.physbeh.2017.04.025. [PMID] 28476286.
2017
Ischemia-responsive protein 94 is a key mediator of ischemic neuronal injury-induced microglial activation.
Journal of neurochemistry. 142(6):908-919 [DOI] 10.1111/jnc.14111. [PMID] 28640931.
2017
Oxytocin receptors are expressed on dopamine and glutamate neurons in the mouse ventral tegmental area that project to nucleus accumbens and other mesolimbic targets.
The Journal of comparative neurology. 525(5):1094-1108 [DOI] 10.1002/cne.24116. [PMID] 27615433.
2017
Post-stroke angiotensin II type 2 receptor activation provides long-term neuroprotection in aged rats.
PloS one. 12(7) [DOI] 10.1371/journal.pone.0180738. [PMID] 28671997.
2017
Targeting psychologic stress signaling pathways in Alzheimer’s disease.
Molecular neurodegeneration. 12(1) [DOI] 10.1186/s13024-017-0190-z. [PMID] 28633663.
2016
Angiotensin type 1a receptors in the paraventricular nucleus of the hypothalamus control cardiovascular reactivity and anxiety-like behavior in male mice.
Physiological genomics. 48(9):667-76 [DOI] 10.1152/physiolgenomics.00029.2016. [PMID] 27468749.
2016
Angiotensin Type-2 Receptors Influence the Activity of Vasopressin Neurons in the Paraventricular Nucleus of the Hypothalamus in Male Mice.
Endocrinology. 157(8):3167-80 [DOI] 10.1210/en.2016-1131. [PMID] 27267713.
2016
Angiotensin-converting enzyme 2 inhibits high-mobility group box 1 and attenuates cardiac dysfunction post-myocardial ischemia.
Journal of molecular medicine (Berlin, Germany). 94(1):37-49 [PMID] 26498282.
View on: PubMed
2016
Conditioned stress prevents cue-primed cocaine reinstatement only in stress-responsive rats.
Stress (Amsterdam, Netherlands). 19(4):406-18 [DOI] 10.1080/10253890.2016.1189898. [PMID] 27181613.
2016
Cross talk between AT1 receptors and Toll-like receptor 4 in microglia contributes to angiotensin II-derived ROS production in the hypothalamic paraventricular nucleus.
American journal of physiology. Heart and circulatory physiology. 310(3):H404-15 [DOI] 10.1152/ajpheart.00247.2015. [PMID] 26637556.
2016
Increasing brain angiotensin converting enzyme 2 activity decreases anxiety-like behavior in male mice by activating central Mas receptors.
Neuropharmacology. 105:114-123 [DOI] 10.1016/j.neuropharm.2015.12.026. [PMID] 26767952.
2016
Reporter mouse strain provides a novel look at angiotensin type-2 receptor distribution in the central nervous system.
Brain structure & function. 221(2):891-912 [DOI] 10.1007/s00429-014-0943-1. [PMID] 25427952.
2015
Adipocyte glucocorticoid receptors mediate fat-to-brain signaling.
Psychoneuroendocrinology. 56:110-9 [DOI] 10.1016/j.psyneuen.2015.03.008. [PMID] 25808702.
2015
Angiotensin type 2 receptors: blood pressure regulation and end organ damage.
Current opinion in pharmacology. 21:115-21 [DOI] 10.1016/j.coph.2015.01.004. [PMID] 25677800.
2015
Hydration and beyond: neuropeptides as mediators of hydromineral balance, anxiety and stress-responsiveness.
Frontiers in systems neuroscience. 9 [DOI] 10.3389/fnsys.2015.00046. [PMID] 25873866.
2015
Role of neurons and glia in the CNS actions of the renin-angiotensin system in cardiovascular control.
American journal of physiology. Regulatory, integrative and comparative physiology. 309(5):R444-58 [DOI] 10.1152/ajpregu.00078.2015. [PMID] 26084692.
2014
Acute hypernatremia promotes anxiolysis and attenuates stress-induced activation of the hypothalamic-pituitary-adrenal axis in male mice.
Physiology & behavior. 136:91-6 [DOI] 10.1016/j.physbeh.2014.03.027. [PMID] 24704193.
2014
Role of paraventricular nucleus-projecting norepinephrine/epinephrine neurons in acute and chronic stress.
The European journal of neuroscience. 39(11):1903-11 [DOI] 10.1111/ejn.12587. [PMID] 24766138.
2013
Acute hypernatremia exerts an inhibitory oxytocinergic tone that is associated with anxiolytic mood in male rats.
Endocrinology. 154(7):2457-67 [DOI] 10.1210/en.2013-1049. [PMID] 23653461.
2013
Angiotensin type 1a receptors in the paraventricular nucleus of the hypothalamus protect against diet-induced obesity.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 33(11):4825-33 [DOI] 10.1523/JNEUROSCI.3806-12.2013. [PMID] 23486953.
2013
Neuroimmune communication in hypertension and obesity: a new therapeutic angle?
Pharmacology & therapeutics. 138(3):428-40 [DOI] 10.1016/j.pharmthera.2013.02.005. [PMID] 23458610.
2013
Smooth muscle LDL receptor-related protein-1 deletion induces aortic insufficiency and promotes vascular cardiomyopathy in mice.
PloS one. 8(11) [DOI] 10.1371/journal.pone.0082026. [PMID] 24312398.
2012
Amylin blunts hyperphagia and reduces weight and fat gain during recovery in socially stressed rats.
American journal of physiology. Regulatory, integrative and comparative physiology. 303(6):R676-82 [DOI] 10.1152/ajpregu.00090.2012. [PMID] 22832535.
2012
Identification of chronic stress-activated regions reveals a potential recruited circuit in rat brain.
The European journal of neuroscience. 36(4):2547-55 [DOI] 10.1111/j.1460-9568.2012.08161.x. [PMID] 22789020.
2011
Blood-borne angiotensin II acts in the brain to influence behavioral and endocrine responses to psychogenic stress.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 31(42):15009-15 [DOI] 10.1523/JNEUROSCI.0892-11.2011. [PMID] 22016534.
2011
Central angiotensin II has catabolic action at white and brown adipose tissue.
American journal of physiology. Endocrinology and metabolism. 301(6):E1081-91 [DOI] 10.1152/ajpendo.00307.2011. [PMID] 21862725.
2011
Central melanocortins modulate mesocorticolimbic activity and food seeking behavior in the rat.
Physiology & behavior. 102(5):491-5 [DOI] 10.1016/j.physbeh.2010.12.017. [PMID] 21172367.
2011
Hydration state controls stress responsiveness and social behavior.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 31(14):5470-6 [DOI] 10.1523/JNEUROSCI.6078-10.2011. [PMID] 21471383.
2011
Opposing effects of chronic stress and weight restriction on cardiovascular, neuroendocrine and metabolic function.
Physiology & behavior. 104(2):228-34 [DOI] 10.1016/j.physbeh.2011.03.002. [PMID] 21396386.
2010
Acute exposure to a high-fat diet alters meal patterns and body composition.
Physiology & behavior. 99(1):33-9 [DOI] 10.1016/j.physbeh.2009.10.004. [PMID] 19835896.
2010
Glucose parameters are altered in mouse offspring produced by assisted reproductive technologies and somatic cell nuclear transfer.
Biology of reproduction. 83(2):220-7 [DOI] 10.1095/biolreprod.109.082826. [PMID] 20445127.
2010
Meal patterns and hypothalamic NPY expression during chronic social stress and recovery.
American journal of physiology. Regulatory, integrative and comparative physiology. 299(3):R813-22 [DOI] 10.1152/ajpregu.00820.2009. [PMID] 20610828.
2010
Nongenomic actions of adrenal steroids in the central nervous system.
Journal of neuroendocrinology. 22(8):846-61 [DOI] 10.1111/j.1365-2826.2010.02000.x. [PMID] 20367759.
2010
Pleasurable behaviors reduce stress via brain reward pathways.
Proceedings of the National Academy of Sciences of the United States of America. 107(47):20529-34 [DOI] 10.1073/pnas.1007740107. [PMID] 21059919.
2010
Post-ingestive signals and satiation of water and sodium intake of male rats.
Physiology & behavior. 99(5):657-62 [DOI] 10.1016/j.physbeh.2010.01.030. [PMID] 20138075.
2010
The renin angiotensin system and the metabolic syndrome.
Physiology & behavior. 100(5):525-34 [DOI] 10.1016/j.physbeh.2010.03.018. [PMID] 20381510.
2009
Dominant rats are natural risk takers and display increased motivation for food reward.
Neuroscience. 162(1):23-30 [DOI] 10.1016/j.neuroscience.2009.04.039. [PMID] 19393296.
2009
Sex differences in physiology and behavior: focus on central actions of ovarian hormones.
Physiology & behavior. 97(2):141-2 [DOI] 10.1016/j.physbeh.2009.02.026. [PMID] 19268482.
2009
The effect of angiotensin-converting enzyme inhibition using captopril on energy balance and glucose homeostasis.
Endocrinology. 150(9):4114-23 [DOI] 10.1210/en.2009-0065. [PMID] 19497971.
2008
Angiotensin type 1 receptors in the subfornical organ mediate the drinking and hypothalamic-pituitary-adrenal response to systemic isoproterenol.
Endocrinology. 149(12):6416-24 [DOI] 10.1210/en.2008-0477. [PMID] 18687780.
2007
Oestrogen affects the cardiovascular and central responses to isoproterenol of female rats.
The Journal of physiology. 582(Pt 1):435-47 [PMID] 17430989.
View on: PubMed
2007
Richter and sodium appetite: from adrenalectomy to molecular biology.
Appetite. 49(2):353-67 [PMID] 17561308.
View on: PubMed
2006
Oestrogen and weight loss decrease isoproterenol-induced Fos immunoreactivity and angiotensin type 1 mRNA in the subfornical organ of female rats.
The Journal of physiology. 573(Pt 1):251-62 [PMID] 16543266.
View on: PubMed
2004
Gestational and early postnatal dietary NaCl levels affect NaCl intake, but not stimulated water intake, by adult rats.
American journal of physiology. Regulatory, integrative and comparative physiology. 286(6):R1043-50 [PMID] 14764435.
View on: PubMed
2003
Cardiovascular function and circadian patterns in rats after area postrema lesions or prolonged food restriction.
Neuroscience letters. 350(1):46-50 [PMID] 12962914.
View on: PubMed
2003
Estrogen influences stimulated water intake by ovariectomized female rats.
Physiology & behavior. 79(2):267-74 [PMID] 12834798.
View on: PubMed
2002
Fos expression in non-catecholaminergic neurons in medullary and pontine nuclei after volume depletion induced by polyethylene glycol.
Brain research. 948(1-2):149-54 [PMID] 12383967.
View on: PubMed
2001
Altered NaCl taste responses precede increased NaCl ingestion during Na(+) deprivation.
Physiology & behavior. 72(5):743-9 [PMID] 11337007.
View on: PubMed
2001
Expression of Ca2+/calmodulin-dependent protein kinase II delta-subunit isoforms in rats with hypertensive cardiac hypertrophy.
Molecular and cellular biochemistry. 220(1-2):69-76 [PMID] 11451385.
View on: PubMed
2000
[A comparative study of cardiac function in transgenic hypertensive rats, in spontaneously hypertensive rats and in normotensive rats].
Archives des maladies du coeur et des vaisseaux. 93(8):993-6 [PMID] 10989744.
View on: PubMed
2000
A role for a helical connector between two receptor binding sites of a long-chain peptide hormone.
The Journal of biological chemistry. 275(8):5702-9 [PMID] 10681555.
View on: PubMed

Grants

Jul 2021 ACTIVE
Seven tesla preclinical MRI/S scanner for structural, functional and molecular imaging
Role: Other
Funding: NATL INST OF HLTH OD
Jan 2021 ACTIVE
Central Nervous System Plasticity in Airway Disease
Role: Co-Investigator
Funding: NATL INST OF HLTH NHLBI
Feb 2020 ACTIVE
Interrogating stress-relieving neural circuits to alleviate cardiovascular disease
Role: Principal Investigator
Funding: NATL INST OF HLTH NHLBI
Aug 2019 ACTIVE
Immunotherapy targeting the HPA axis in Alzheimers disease
Role: Co-Investigator
Funding: NATL INST OF HLTH NIA
Mar 2019 ACTIVE
Crosstalk between dopamine and glucocorticoids in high levels of nicotine intake and anhedonia in rats
Role: Co-Investigator
Funding: NATL INST OF HLTH NIDA
Jan 2019 ACTIVE
Interrogating distinct angiotensin type-1 and type-2 receptor containing brain circuits to understand and alleviate hypertension
Role: Co-Investigator
Funding: NATL INST OF HLTH NHLBI
Jul 2018 – Jun 2020
Ethanol dysregulation of oxytocin-mediated reward
Role: Principal Investigator
Funding: NATL INST OF HLTH NIAAA
Dec 2017 ACTIVE
Neurons expressing angiotensin type 2 receptors in the NTS as an access point for cardiovascular control.
Role: Principal Investigator
Funding: NATL INST OF HLTH NHLBI
Dec 2017 – Jan 2020
Targeting brain angiotensin signaling to discern and alleviate stress-related disease.
Role: Principal Investigator
Funding: NATL INST OF HLTH NHLBI
Aug 2016 – Aug 2019
OR-DRPD-ROF2016: A Neurodevelopmental Theory of Social and Environmental Isolation in Autism
Role: Co-Investigator
Funding: UF RESEARCH
Jun 2016 – Feb 2019
Optogenetic control of neuronal pathways that mediate repetitive behavior
Role: Co-Investigator
Funding: NATL INST OF HLTH NIMH
Apr 2016 ACTIVE
Brain-Gut Microbiome-Immune Axis in Hypertension
Role: Project Manager
Funding: NATL INST OF HLTH NHLBI
Dec 2014 – Jan 2020
Central Mechanisms Underlying the Stress Dampening Effects of Acute Hypernatremia
Role: Principal Investigator
Funding: NATL INST OF HLTH NHLBI
Jul 2013 – May 2019
Angiotensin and neuroimmune activation in hypertension
Role: Project Manager
Funding: NATL INST OF HLTH NHLBI

Education

Ph.D.
2005 · Florida State University
M.S.
2002 · Florida State University
B.A.
1999 · Hiram College

Teaching Profile

Courses Taught
2016-2018,2020-2021
PHA5878C Pt Care 3: Cv and Pulm
2012,2014-2020
PHA7980 Research for Doctoral Dissertation
2012-2020
PHA7979 Advanced Research
2012,2014-2020,2020-2021
PHA6521C Research Techniques in Pharmacodynamics
2012-2021
PHA6512L Experiential Research Training in Pharmacodynamics
2011-2013,2015-2021,2020-2021
PHA6936 Advanced Topics in Pharmaceutical Sciences
2019-2021
PHA6935 Selected Topics in Pharmacy
2012,2020
PHA6971 Research for Master’s Thesis
2012,2015-2020,2020
PHA6910 Supervised Research
2015-2018
PHA5560 Pathphys-Pt Assess I
2018
PHA5784C Pt Care 4: GI and Renal
2012-2013,2016
PHA5902 Research Pharmacodyna
2016
PHA4911 Undergraduate Research in Pharmacodynamics
2015
GMS7794 Neuroscience Seminar
2012-2015
PHA5561C Physiol Bas Disease 2
2013-2015
PHA6509 Systems Physiology and Pathophysiology II
2014
PHA5560C Physiol Bas Disease 1
2011,2013-2014
PHA7939 Journal Colloquy in Pharmacodynamics
2014
PHA6508 Systems Physiology and Pathophysiology I
2014
PHA6938 Research Seminar
2021
PHA6894 Introduction to Graduate Studies

Contact Details

Phones:
Business:
(352) 273-6977
Emails: