Siva Rama Raju Kanumuri

Siva Rama Raju Kanumuri, Ph.D.

Assistant Scientist

Department: Pharmaceutics
Business Phone: (513) 344-0547
Business Email: kanumuris@ufl.edu

About Siva Rama Raju Kanumuri

Siva Rama Raju Kanumuri is an Assistant Scientist in Department of Pharmaceutics and CTSI-Translational Drug Development Core at University of Florida, Gainesville. He received his bachelor’s degree in Pharmaceutical Sciences from Andhra University, India in 2008 and later received his Master of Science (M.S.) in Pharmaceutics from National Institute of Pharmaceutical Education and Research, Raebareli, India in 2011 with a MHRD-Post-graduate fellowship by All India Council for Technical Education (AICTE), India. After his Master’s, he worked as a project associate for 2 years in CSIR- Central Drug Research Institute, India and later joined as CSIR- Senior Research Fellowship (2013-2016) to pursue graduation (Ph.D.) from the same institute. After his graduate studies, Dr. Raju moved to US and joined as a postdoctoral fellow under the supervision of Professor Giovanni M. Pauletti at the University of Cincinnati (2017-2019) and later under the supervision of Dr. Christopher McCurdy at the University of Florida (2019-2022).

Accomplishments

International Travel Award
2015 · Centre for International Co-operation in Science (India)
International Travel Award to attend AAPS Conference
2015 · Department of Science and Technology, New Delhi (India)
ISSX Travel Award
2015 · International Society for the Study of Xenobiotics (ISSX) Organizing committee
CSIR- Senior Research Fellowship
2013-2016 · Council for Scientific and Industrial Research (CSIR), India
8th Dr. P.D. Sethi Annual Award 2012 for Best Paper in Pharmaceutical Analysis
2012 · Anchrom Pvt Ltd (India)
Silver Medal in M.S (Pharmaceutics)
2011 · National Institute of Pharmaceutical Education and Research (NIPER), Raebareli, India
MHRD-Post-graduate fellowship
2009-2011 · All India Council for Technical Education, India

Research Profile

Dr. Siva Rama Raju Kanumuri is a pharmacokinetics scientist with more than 10 years of research experience in small molecule preclinical discovery ADMET to clinical pharmacokinetic studies. His research work focusses on designing and orchestrating the execution of various in vitro and in vivo pharmacokinetic studies (human, rodents, and non-rodents) for optimization of ADMET properties of new chemical entities (NCE), drugs under development and natural products. His research helps identify small molecules to move forward in drug development based on metabolic stability, metabolite identification, formation of reactive metabolites and drug transport and permeability across biological barriers.

Open Researcher and Contributor ID (ORCID)

0000-0002-6087-6587

Areas of Interest
  • Bioanalysis
  • Drug Metabolism
  • PK-PD Modelling
  • Pharmacokinetic Modeling and Simulation
  • Pharmacokinetics/pharmacodynamics
  • Population pharmacokinetics

Publications

2024
DR5 disulfide bonding as a sensor and effector of protein folding stress.
bioRxiv : the preprint server for biology. [DOI] 10.1101/2024.03.04.583390. [PMID] 38496520.
2024
Effects of kratom on driving: Results from a cross-sectional survey, ecological momentary assessment, and pilot simulated driving Study.
Traffic injury prevention. 25(4):594-603 [DOI] 10.1080/15389588.2024.2327827. [PMID] 38497810.
2024
Explorations of Agonist Selectivity for the α9* nAChR with Novel Substituted Carbamoyl/Amido/Heteroaryl Dialkylpiperazinium Salts and Their Therapeutic Implications in Pain and Inflammation.
Journal of medicinal chemistry. 67(11):8642-8666 [DOI] 10.1021/acs.jmedchem.3c02429. [PMID] 38748608.
2024
Pharmacokinetic Interaction of Kratom and Cannabidiol in Male Rats
Pharmaceutics. 16(3) [DOI] 10.3390/pharmaceutics16030318. [PMID] 38543213.
2024
Preclinical pharmacokinetic studies of villocarine A, an active Uncaria alkaloid.
Drug testing and analysis. [DOI] 10.1002/dta.3703. [PMID] 38747129.
2024
Responses to a “Typical” Morning Dose of Kratom in People Who Use Kratom Regularly: A Direct-Observation Study
Journal of Addiction Medicine. 18(2):144-152 [DOI] 10.1097/adm.0000000000001259.
2023
An in vitro evaluation of kratom (Mitragyna speciosa) on the catalytic activity of carboxylesterase 1 (CES1)
Chemico-Biological Interactions. 384 [DOI] 10.1016/j.cbi.2023.110715. [PMID] 37716419.
2023
Comparative Pharmacokinetics of Commercially Available Cannabidiol Isolate, Broad-Spectrum, and Full-Spectrum Products.
European journal of drug metabolism and pharmacokinetics. 48(4):427-435 [DOI] 10.1007/s13318-023-00839-3. [PMID] 37337087.
2023
Metabolite and Molecular Characterization of Mitragyna speciosa Identifies Developmental and Genotypic Effects on Monoterpene Indole and Oxindole Alkaloid Composition
Journal of Natural Products. 86(4):1042-1052 [DOI] 10.1021/acs.jnatprod.3c00092.
2023
Oral IRAK-4 Inhibitor CA-4948 Is Blood-Brain Barrier Penetrant and Has Single-Agent Activity against CNS Lymphoma and Melanoma Brain Metastases
Clinical Cancer Research. 29(9):1751-1762 [DOI] 10.1158/1078-0432.ccr-22-1682.
2023
Pharmacokinetic and Pharmacodynamic Consequences of Cytochrome P450 3A Inhibition on Mitragynine Metabolism in Rats
Journal of Pharmacology and Experimental Therapeutics. 385(3):180-192 [DOI] 10.1124/jpet.122.001525. [PMID] 37019472.
2022
Clinical pharmacokinetics of kavalactones after oral dosing of standardized kava extract in healthy volunteers
Journal of Ethnopharmacology. 297 [DOI] 10.1016/j.jep.2022.115514. [PMID] 35777607.
2022
Metabolism of Speciociliatine, an Overlooked Kratom Alkaloid for its Potential Pharmacological Effects.
The AAPS journal. 24(5) [DOI] 10.1208/s12248-022-00736-8. [PMID] 35854066.
2022
The Lack of Contribution of 7-Hydroxymitragynine to the Antinociceptive Effects of Mitragynine in Mice: A Pharmacokinetic and Pharmacodynamic Study
Drug Metabolism and Disposition. 50(2):158-167 [DOI] 10.1124/dmd.121.000640. [PMID] 34759012.
2022
UPLC-MS/MS method for the quantification of MCI-77, a novel sigma-1 receptor ligand, and its application to pharmacokinetic studies
Journal of Chromatography B. 1196 [DOI] 10.1016/j.jchromb.2022.123187.
2021
A novel nanosized phospholipid complex of Biochanin A for improving oral bioavailability: Preparation and in-vitro/in-vivo characterizations
Journal of Drug Delivery Science and Technology. 61 [DOI] 10.1016/j.jddst.2020.102254.
2021
Characterization of different forms of kava (Piper methysticum) products by UPLC-MS/MS.
Planta medica. 88(14):1348-1359 [DOI] 10.1055/a-1708-1994. [PMID] 34839465.
2021
Chromatographic separation and estimation of natural antimalarial flavonoids in biological matrices
Proceedings of the Indian National Science Academy. 87(3):446-468 [DOI] 10.1007/s43538-021-00050-5.
2021
Elucidation of plasma protein binding, blood partitioning, permeability, CYP phenotyping and CYP inhibition studies of Withanone using validated UPLC method: An active constituent of neuroprotective herb Ashwagandha.
Journal of ethnopharmacology. 270 [DOI] 10.1016/j.jep.2021.113819. [PMID] 33460762.
2021
Exploring the Chemistry of Alkaloids from Malaysian Mitragyna speciosa (Kratom) and the Role of Oxindoles on Human Opioid Receptors.
Journal of natural products. 84(4):1034-1043 [DOI] 10.1021/acs.jnatprod.0c01055. [PMID] 33635670.
2021
Highly Specific Sigma Receptor Ligands Exhibit Anti-Viral Properties in SARS-CoV-2 Infected Cells
Pathogens. 10(11) [DOI] 10.3390/pathogens10111514. [PMID] 34832669.
2021
Oral Pharmacokinetics in Beagle Dogs of the Mitragynine Metabolite, 7-Hydroxymitragynine.
European journal of drug metabolism and pharmacokinetics. 46(3):459-463 [DOI] 10.1007/s13318-021-00684-2. [PMID] 33847897.
2021
Preclinical pharmacokinetic study of speciociliatine, a kratom alkaloid, in rats using an UPLC-MS/MS method.
Journal of pharmaceutical and biomedical analysis. 194 [DOI] 10.1016/j.jpba.2020.113778. [PMID] 33277117.
2020
Bioanalytical method development and pharmacokinetics of MCI-92, a sigma-1 receptor ligand.
Journal of pharmaceutical and biomedical analysis. 191 [DOI] 10.1016/j.jpba.2020.113610. [PMID] 32971495.
2020
Bioavailability, tissue distribution and excretion studies of a potential anti-osteoporotic agent, medicarpin, in female rats using validated LC-MS/MS method.
Journal of pharmaceutical and biomedical analysis. 180 [DOI] 10.1016/j.jpba.2019.112978. [PMID] 31855725.
2020
Exploration of cytochrome P450 inhibition mediated drug-drug interaction potential of kratom alkaloids.
Toxicology letters. 319:148-154 [DOI] 10.1016/j.toxlet.2019.11.005. [PMID] 31707106.
2020
Metabolism of a Kratom Alkaloid Metabolite in Human Plasma Increases Its Opioid Potency and Efficacy.
ACS pharmacology & translational science. 3(6):1063-1068 [DOI] 10.1021/acsptsci.0c00075. [PMID] 33344889.
2020
Pharmacokinetics and Safety of Mitragynine in Beagle Dogs.
Planta medica. 86(17):1278-1285 [DOI] 10.1055/a-1212-5475. [PMID] 32693425.
2020
Regulatory sampling of industrial hemp plant samples (Cannabis sativa L.) using UPLC-MS/MS method for detection and quantification of twelve cannabinoids.
Journal of cannabis research. 2(1) [DOI] 10.1186/s42238-020-00050-0. [PMID] 33526142.
2019
LC-ESI-MS/MS method for the simultaneous determination of isoformononetin, daidzein, and equol in rat plasma: Application to a preclinical pharmacokinetic study
Journal of Chromatography B. 1129 [DOI] 10.1016/j.jchromb.2019.121776.
2018
Development and validation of UPLC-MS/MS assay for quantification of cladrin: Absolute bioavailability and dose proportionality study in rats
Journal of Pharmaceutical and Biomedical Analysis. 152:289-297 [DOI] 10.1016/j.jpba.2018.01.044.
2018
Metabolic Enhancer Piracetam Attenuates the Translocation of Mitochondrion-Specific Proteins of Caspase-Independent Pathway, Poly [ADP-Ribose] Polymerase 1 Up-regulation and Oxidative DNA Fragmentation
Neurotoxicity Research. 34(2):198-219 [DOI] 10.1007/s12640-018-9878-2.
2018
New therapeutic activity of metabolic enhancer piracetam in treatment of neurodegenerative disease: Participation of caspase independent death factors, oxidative stress, inflammatory responses and apoptosis
Biochimica et Biophysica Acta (BBA) – Molecular Basis of Disease. 1864(6):2078-2096 [DOI] 10.1016/j.bbadis.2018.03.014.
2018
Species differences between rat and human in vitro metabolite profile, in vivo predicted clearance, CYP450 inhibition and CYP450 isoforms that metabolize benzanthrone: Implications in risk assessment
Food and Chemical Toxicology. 111:94-101 [DOI] 10.1016/j.fct.2017.11.009.
2017
Antileishmanial Activity of Pyrazolopyridine Derivatives and Their Potential as an Adjunct Therapy with Miltefosine.
Journal of medicinal chemistry. 60(3):1041-1059 [DOI] 10.1021/acs.jmedchem.6b01447. [PMID] 28059524.
2017
Bioanalysis of antitubercular drugs using liquid chromatography.
Journal of pharmaceutical and biomedical analysis. 134:295-309 [DOI] 10.1016/j.jpba.2016.11.006. [PMID] 27951471.
2017
Pharmacological activation of aldehyde dehydrogenase 2 promotes osteoblast differentiation via bone morphogenetic protein-2 and induces bone anabolic effect
Toxicology and Applied Pharmacology. 316:63-73 [DOI] 10.1016/j.taap.2016.12.013.
2017
RP-HPLC Separation of Isomeric Withanolides: Method Development, Validation and Application to In situ Rat Permeability Determination
Journal of Chromatographic Science. 55(7):729-735 [DOI] 10.1093/chromsci/bmx027.
2017
Synthesis and Evaluation of Chirally Defined Side Chain Variants of 7-Chloro-4-Aminoquinoline To Overcome Drug Resistance in Malaria Chemotherapy
Antimicrobial Agents and Chemotherapy. 61(3) [DOI] 10.1128/aac.01152-16.
2016
Comparative assessment of bioanalytical method validation guidelines for pharmaceutical industry
Journal of Pharmaceutical and Biomedical Analysis. 126:83-97 [DOI] 10.1016/j.jpba.2016.03.052.
2016
DBS-platform for biomonitoring and toxicokinetics of toxicants: proof of concept using LC-MS/MS analysis of fipronil and its metabolites in blood
Scientific Reports. 6(1) [DOI] 10.1038/srep22447. [PMID] 26960908.
2016
Development and validation of an LC-MS/MS method for simultaneous determination of piperaquine and 97-63, the active metabolite of CDRI 97-78, in rat plasma and its application in interaction study
Drug Testing and Analysis. 8(2):221-227 [DOI] 10.1002/dta.1807.
2016
Dietary Isoflavones as Modulators of Drug Metabolizing Enzymes and Transporters: Effect on Prescription Medicines
Critical Reviews in Food Science and Nutrition. 56(sup1):S95-S109 [DOI] 10.1080/10408398.2015.1045968.
2016
Effect of polymorphism on the preclinical pharmacoknetics of desbutyl-lumefantrine, a potential anti-malarial agent
Drug Metabolism Reviews. 48((Suppl 1))
2016
Naturally Occurring Carbazole Alkaloids from Murraya koenigii as Potential Antidiabetic Agents.
Journal of natural products. 79(5):1276-84 [DOI] 10.1021/acs.jnatprod.5b00883. [PMID] 27136692.
2016
Pharmacokinetic endeavors for antimalarial therapeutics
International Journal of Infectious Diseases. 45:130-131 [DOI] 10.1016/j.ijid.2016.02.323.
2016
Preclinical pharmacokinetics and species differences in the CYP-mediated metabolism of isoformononetin, a potential anti-osteoporotic isoflavonoid
Drug Metabolism Reviews. 48(Suppl 1) [DOI] 10.3109/03602532.2015.107193.
2016
Validation of a Rapid and Sensitive UPLC–MS-MS Method Coupled with Protein Precipitation for the Simultaneous Determination of Seven Pyrethroids in 100 µL of Rat Plasma by Using Ammonium Adduct as Precursor Ion
Journal of Analytical Toxicology. 40(3):213-221 [DOI] 10.1093/jat/bkw002. [PMID] 26801239.
2015
Arteether nanoemulsion for enhanced efficacy against Plasmodium yoelii nigeriensis malaria: An approach by enhanced bioavailability
Colloids and Surfaces B: Biointerfaces. 126:467-475 [DOI] 10.1016/j.colsurfb.2014.12.052.
2015
Assessment of pharmacokinetic compatibility of short acting CDRI candidate trioxane derivative, 99–411, with long acting prescription antimalarials, lumefantrine and piperaquine
Scientific Reports. 5(1) [DOI] 10.1038/srep17264. [PMID] 26602250.
2015
Bioavailability, plasma protein binding and metabolic stability studies of a ALDH2 activator, alda-1, using a validated LC-ESI-MS/MS method in rat plasma
RSC Advances. 5(67):54395-54402 [DOI] 10.1039/c5ra06859b.
2015
Dried blood spots for neonates and pediatric populations
New Sampling Strategies in Toxicology and Therapeutic Drug Monitoring. 54-65 [DOI] 10.4155/fseb2013.14.363.
2015
In vivo prediction of CYP-mediated metabolic interaction potential of formononetin and biochanin A using in vitro human and rat CYP450 inhibition data
Toxicology Letters. 239(1):1-8 [DOI] 10.1016/j.toxlet.2015.08.202.
2015
LC-ESI–MS/MS method for bioanalytical determination of osteogenic phytoalexin, medicarpin, and its application to preliminary pharmacokinetic studies in rats
Journal of Chromatography B. 1001:9-16 [DOI] 10.1016/j.jchromb.2015.06.025.
2015
Metabolic enhancer piracetam attenuates rotenone induced oxidative stress: a study in different rat brain regions.
Acta neurobiologiae experimentalis. 75(4):399-411 [DOI] 10.55782/ane-2015-2045. [PMID] 26994419.
2015
No effect on pharmacokinetics of tamoxifen and 4-hydroxytamoxifen by multiple doses of red clover capsule in rats
Scientific Reports. 5(1) [DOI] 10.1038/srep16126. [PMID] 26530625.
2015
Phytochemical analysis of isoflavonoids using liquid chromatography coupled with tandem mass spectrometry
Phytochemistry Reviews. 14(3):469-498 [DOI] 10.1007/s11101-015-9400-x.
2015
Simultaneous determination of centchroman and tamoxifen along with their metabolites in rat plasma using LC–MS/MS
Bioanalysis. 7(8):967-979 [DOI] 10.4155/bio.14.253.
2015
Simultaneous quantification of proposed anti-malarial combination comprising of lumefantrine and CDRI 97–78 in rat plasma using the HPLC–ESI-MS/MS method: application to drug interaction study
Malaria Journal. 14(1) [DOI] 10.1186/s12936-015-0684-5. [PMID] 25895956.
2014
Effect of Red Clover on CYP Expression: An Investigation of Herb-Drug Interaction at Molecular Level.
Indian journal of pharmaceutical sciences. 76(3):261-6 [PMID] 25035541.
2014
Investigation of the Functional Role of P-Glycoprotein in Limiting the Oral Bioavailability of Lumefantrine
Antimicrobial Agents and Chemotherapy. 58(1):489-494 [DOI] 10.1128/aac.01382-13.
2014
Palladium and copper-catalyzed ligand-free coupling of phenylhydrazines in water
RSC Adv.. 4(82):43336-43340 [DOI] 10.1039/c4ra04621h.
2014
Pharmacokinetics study of arteether loaded solid lipid nanoparticles: An improved oral bioavailability in rats
International Journal of Pharmaceutics. 466(1-2):321-327 [DOI] 10.1016/j.ijpharm.2014.03.036.
2014
Self-nanoemulsifying drug delivery systems (SNEDDS) for oral delivery of arteether: pharmacokinetics, toxicity and antimalarial activity in mice
RSC Adv.. 4(110):64905-64918 [DOI] 10.1039/c4ra09267h.
2014
The metabolic enhancer piracetam attenuates mitochondrion-specific endonuclease G translocation and oxidative DNA fragmentation
Free Radical Biology and Medicine. 73:278-290 [DOI] 10.1016/j.freeradbiomed.2014.05.014.
2013
Bioanalysis of antimalarials using liquid chromatography
TrAC Trends in Analytical Chemistry. 42:186-204 [DOI] 10.1016/j.trac.2012.09.014.
2013
Disposition of Pharmacologically Active Dietary Isoflavones in Biological Systems
Current Drug Metabolism. 14(4):369-380 [DOI] 10.2174/1389200211314040002.
2013
Dried blood spots in bioanalysis of antimalarials: relevance and challenges in quantitative assessment of antimalarial drugs
Bioanalysis. 5(17):2171-2186 [DOI] 10.4155/bio.13.180.
2013
Nanotechnology in Modern Medical Imaging and Interventions
Book Chapter.
2013
Synthesis and Antimalarial Activity of 3,3-Spiroanellated 5,6-Disubstituted 1,2,4-Trioxanes
ACS Medicinal Chemistry Letters. 4(2):165-169 [DOI] 10.1021/ml300188t. [PMID] 24900640.
2013
Synthesis and insight into the structure-activity relationships of chalcones as antimalarial agents.
Journal of medicinal chemistry. 56(1):31-45 [DOI] 10.1021/jm300588j. [PMID] 23270565.
2013
Utility of noninvasive biomatrices in pharmacokinetic studies
Biomedical Chromatography. 27(10):1354-1366 [DOI] 10.1002/bmc.2996.
2012
Fate of pharmacologically active isoflavones upon administration in sprague dawley rats
J Bioavailability and Bioequivalence. 4(3)
2012
Reduced Bioavailability of Tamoxifen and its Metabolite 4-Hydroxytamoxifen After Oral Administration with Biochanin A (an Isoflavone) in Rats
Phytotherapy Research. 26(2):303-307 [DOI] 10.1002/ptr.3652.
2012
Simultaneous determination of nine model compounds in permeability samples using RP-HPLC: Application to prove the cassette administration principle in single pass intestinal perfusion study in rats
Journal of Pharmaceutical and Biomedical Analysis. 67-68:71-76 [DOI] 10.1016/j.jpba.2012.03.048.
2011
Intravenous pharmacokinetics, oral bioavailability, dose proportionality and in situ permeability of anti-malarial lumefantrine in rats
Malaria Journal. 10(1) [DOI] 10.1186/1475-2875-10-293. [PMID] 21985153.
2010
Comparative study on various tableting properties of Phenytoin sodium mouth dissolving dosage form with and without spherical crystals
Int J of Pharm Sci Tech. 5(2):19-26
2010
Nanosuspension: an attempt to enhance bioavailability of poorly soluble drugs
International Journal of Pharmaceutical Sciences and Research. 1(9):1-11
2010
NANOVEHICLES: AN EFFICIENT CARRIER FOR ACTIVE MOLECULES FOR ENTRY INTO THE CELL
Int J Pharm Sci Rev Res. 4(3)

Education

PG Diploma in Patent Law
2014-2015 · NALSAR University of Law, India
PhD
2013-2016 · CSIR-Central Drug Research Institute/ Academy of Scientific and Innovative Research, India
M.S. (Pharmaceutics)
2009-2011 · Department of Pharmaceutics, National Institute of Pharmaceutical Education & Research (NIPER), India
Bachelor of Pharmacy
2004-2008 · Andhra University, India

Contact Details

Phones:
Business:
(513) 344-0547
Emails:
Business:
kanumuris@ufl.edu
Addresses:
Business Mailing:
PO Box 100494
GAINESVILLE FL 32610