Margaret O James, Ph.D.
Professor Emeritus
About Margaret O James
Margaret James, Ph.D., investigates factors affecting the metabolism and toxicity of drugs and other xenobiotics in humans and animal species, including aquatic species. She was chosen as the Jack C Massey Professor of Pharmacy for the term 2006-2021, as a University of Florida Research Foundation Professor, 1997-2000, and as a UF Term professor 2018-2021. Her laboratory studies mechanisms of uptake, biotransformation, excretion and toxicity of xenobiotics, especially environmental chemicals. Dr. James also investigates the influence of xenobiotics on the metabolism of steroid hormones.
Additional Positions:
Study section member
2019 – 2023
·
National Institutes of Health
Accomplishments
Fellow
2011
·
American Association for the Advancement of Science
Teaching Profile
Courses Taught
2015-2021
PHA5439 Prin Med Chem-Pcol I
2009-2021
PHA7979 Advanced Research
2008-2020
PHA7980 Research for Doctoral Dissertation
2006-2023
PHA6425 Drug Biotrans and Molecular Mechanisms of Toxicity
2008,2010-2012,2012-2015,2014-2021,2017-2022,2020-2022
PHA6447 Drug Design
2010-2012,2015-2020,2020
PHA6971 Research for Master’s Thesis
2014-2022
PHA6905C Research Procedures in Medicinal Chemistry
2014-2015,2017-2020,2019-2022
GMS6065 Fundamentals of Cancer Biology
2015-2016,2018-2021,2020-2022
GMS6009 Principles of Drug Action and Therapeutics
2015,2018-2020,2020-2022
PHA6935 Selected Topics in Pharmacy
2019,2021
PHA5732 Year 1 Enhancement
2017-2018
GMS6847 Translational Research and Therapeutics: Bench, Bedside, Community, & Policy
2018
CHM7979 Advanced Research
2018
CHM7980 Research for Doctoral Dissertation
2018
VME7980 Research for Doctoral Dissertation
2018
GMS7979 Advanced Research
2017
PHA5933 Select Top Pharmacy
2011-2016
PHA6910 Supervised Research
2012-2015
PHA5436 Struc/Func Nucleotide
2007,2010-2014
PHA5433 Funda Medicinal Chem
2009-2011,2013
PHA5906 Research in Med Chem
2008-2010
PHA4906 Probs Medicinal Chem
2006
PHA4933 Select Top Pharmacy
2021
PHA5787 Pharmacotherapy 5
2021
PHA5787C Pt Care 5: Endo
2024
PHA6468 Biotransformation Considerations in Drug Design
Research Profile
Drug Biotransformation and Toxicology: Role of glutathione transferase zeta in the metabolism of dichloroacetic acid.
Dichloroacetate is both an orphan drug, used to reverse the Warburg effect in cancer cells and to treat acquired and congenital lactic acidosis, and an environmental pollutant found in chlorinated drinking water. The first step in metabolism of dichloroacetate is glutathione-dependent dehalogenation to glyoxylate, catalyzed by GSTZ1, an enzyme whose physiological function is in isomerization of tyrosine catabolites. As well as the studying the biotransformation of dichloroacetate, we are interested in understanding the toxicological effects of exposure to this agent.
Publications
Academic Articles
2022
Drug Metabolism: Phase II Enzymes
Comprehensive Pharmacology.
563-584
[DOI] 10.1016/b978-0-12-820472-6.00123-7.
2022
Editorial: Translational Research and Drug Repurposing for Non-Communicable Diseases (NCDs)
Frontiers in Pharmacology.
13
[DOI] 10.3389/fphar.2022.879611.
[PMID] 35387337.
2022
Hepatic demethylation of methoxy-bromodiphenyl ethers and conjugation of the resulting hydroxy-bromodiphenyl ethers in a marine fish, the red snapper, Lutjanus campechanus, and a freshwater fish, the channel catfish, Ictalurus punctatus.
Chemosphere.
286(Pt 1)
[DOI] 10.1016/j.chemosphere.2021.131620.
[PMID] 34303902.
2021
Enzyme Kinetics of PAPS-Sulfotransferase.
Methods in molecular biology (Clifton, N.J.).
2342:285-300
[DOI] 10.1007/978-1-0716-1554-6_11.
[PMID] 34272699.
2021
Pharmacokinetic and Biochemical Profiling of Sodium Dichloroacetate in Pregnant Ewes and Fetuses
Drug Metabolism and Disposition.
49(6):451-458
[DOI] 10.1124/dmd.120.000330.
[PMID] 33811107.
2020
Age-Related Changes in miRNA Expression Influence GSTZ1 and Other Drug Metabolizing Enzymes
Drug Metabolism and Disposition.
48(7):563-569
[DOI] 10.1124/dmd.120.090639.
[PMID] 32357971.
2020
Effects of Multiple Doses of Dichloroacetate on GSTZ1 Expression and Activity in Liver and Extrahepatic Tissues of Young and Adult Rats
Drug Metabolism and Disposition.
48(11):1217-1223
[DOI] 10.1124/dmd.120.000142.
[PMID] 32873593.
2020
Efficacy data of halogenated phenazine and quinoline agents and an NH125 analogue to veterinary mycoplasmas.
BMC veterinary research.
16(1)
[DOI] 10.1186/s12917-020-02324-4.
[PMID] 32252763.
2020
Exposure of Rats to Multiple Oral Doses of Dichloroacetate Results in Upregulation of Hepatic Glutathione Transferases and NAD(P)H Dehydrogenase [Quinone] 1
Drug Metabolism and Disposition.
48(11):1224-1230
[DOI] 10.1124/dmd.120.000143.
[PMID] 32873592.
2019
Dichloroacetate-induced peripheral neuropathy.
International review of neurobiology.
145:211-238
[DOI] 10.1016/bs.irn.2019.05.003.
[PMID] 31208525.
2019
Mitochondrial Glutathione Transferase Zeta 1 Is Inactivated More Rapidly by Dichloroacetate than the Cytosolic Enzyme in Adult and Juvenile Rat Liver.
Chemical research in toxicology.
32(10):2042-2052
[DOI] 10.1021/acs.chemrestox.9b00207.
[PMID] 31524376.
2019
Phase II metabolism of betulin by rat and human UDP-glucuronosyltransferases and sulfotransferases.
Chemico-biological interactions.
302:190-195
[DOI] 10.1016/j.cbi.2019.02.009.
[PMID] 30776358.
2019
Sulfonation and glucuronidation of hydroxylated bromodiphenyl ethers in human liver.
Chemosphere.
226:132-139
[DOI] 10.1016/j.chemosphere.2019.03.103.
[PMID] 30925405.
2019
Turning the Tide against Antibiotic Resistance by Evaluating Novel, Halogenated Phenazine, Quinoline, and NH125 Compounds against Ureaplasma Species Clinical Isolates and Mycoplasma Type Strains.
Antimicrobial agents and chemotherapy.
63(3)
[DOI] 10.1128/AAC.02265-18.
[PMID] 30642935.
2018
Administration of low dose triclosan to pregnant ewes results in placental uptake and reduced estradiol sulfotransferase activity in fetal liver and placenta.
Toxicology letters.
294:116-121
[DOI] 10.1016/j.toxlet.2018.05.014.
[PMID] 29772265.
2018
Age-Related Changes in Expression and Activity of Human Hepatic Mitochondrial Glutathione Transferase Zeta1.
Drug metabolism and disposition: the biological fate of chemicals.
46(8):1118-1128
[DOI] 10.1124/dmd.118.081810.
[PMID] 29853471.
2018
Model Informed Dose Optimization of Dichloroacetate for the Treatment of Congenital Lactic Acidosis in Children.
Journal of clinical pharmacology.
58(2):212-220
[DOI] 10.1002/jcph.1009.
[PMID] 28914978.
2018
Regulation of dichloroacetate biotransformation in rat liver and extrahepatic tissues by GSTZ1 expression and chloride concentration.
Biochemical pharmacology.
152:236-243
[DOI] 10.1016/j.bcp.2018.04.001.
[PMID] 29626439.
2017
A Mechanism-Based Pharmacokinetic Enzyme Turnover Model for Dichloroacetic Acid Autoinhibition in Rats.
Journal of pharmaceutical sciences.
106(5):1396-1404
[DOI] 10.1016/j.xphs.2017.01.032.
[PMID] 28163135.
2017
A multi-year study of hepatic biomarkers in coastal fishes from the Gulf of Mexico after the Deepwater Horizon Oil Spill.
Marine environmental research.
129:57-67
[DOI] 10.1016/j.marenvres.2017.04.015.
[PMID] 28487163.
2017
Celecoxib affects estrogen sulfonation catalyzed by several human hepatic sulfotransferases, but does not stimulate 17-sulfonation in rat liver.
The Journal of steroid biochemistry and molecular biology.
172:46-54
[DOI] 10.1016/j.jsbmb.2017.05.012.
[PMID] 28552400.
2017
Therapeutic applications of dichloroacetate and the role of glutathione transferase zeta-1.
Pharmacology & therapeutics.
170:166-180
[DOI] 10.1016/j.pharmthera.2016.10.018.
[PMID] 27771434.
2016
Differences in the carcinogenic evaluation of glyphosate between the International Agency for Research on Cancer (IARC) and the European Food Safety Authority (EFSA).
Journal of epidemiology and community health.
70(8):741-5
[DOI] 10.1136/jech-2015-207005.
[PMID] 26941213.
2016
Genomic Effect of Triclosan on the Fetal Hypothalamus: Evidence for Altered Neuropeptide Regulation.
Endocrinology.
157(7):2686-97
[DOI] 10.1210/en.2016-1080.
[PMID] 27145008.
2016
GSTZ1 expression and chloride concentrations modulate sensitivity of cancer cells to dichloroacetate.
Biochimica et biophysica acta.
1860(6):1202-10
[DOI] 10.1016/j.bbagen.2016.01.024.
[PMID] 26850694.
2016
Pharmacogenetic considerations with dichloroacetate dosing.
Pharmacogenomics.
17(7):743-53
[DOI] 10.2217/pgs-2015-0012.
[PMID] 27143230.
2015
Altered Biological Processes in the Late Gestation Ovine Fetal Hypothalamus After Fetal Or Maternal Parenteral Exposure To Triclosan
Reproductive Sciences.
22(1)
2015
Celecoxib influences steroid sulfonation catalyzed by human recombinant sulfotransferase 2A1.
The Journal of Steroid Biochemistry and Molecular Biology.
152:101-13
[DOI] 10.1016/j.jsbmb.2015.05.003.
[PMID] 25960318.
2015
Chloride concentrations in human hepatic cytosol and mitochondria are a function of age.
Biochemical and biophysical research communications.
459(3):463-8
[DOI] 10.1016/j.bbrc.2015.02.128.
[PMID] 25748576.
2015
The influence of human GSTZ1 gene haplotype variations on GSTZ1 expression.
Pharmacogenetics and genomics.
25(5):239-45
[DOI] 10.1097/FPC.0000000000000129.
[PMID] 25738370.
2014
Chloride and other anions inhibit dichloroacetate-induced inactivation of human liver GSTZ1 in a haplotype-dependent manner.
Chemico-biological interactions.
215:33-9
[DOI] 10.1016/j.cbi.2014.02.015.
[PMID] 24632415.
2014
Enzyme kinetics of conjugating enzymes: PAPS sulfotransferase.
Methods in molecular biology (Clifton, N.J.).
1113:187-201
[DOI] 10.1007/978-1-62703-758-7_10.
[PMID] 24523114.
2014
Intracellular Chloride Concentration and Its Impact On Dichloroacetate Metabolism
The FASEB's Journal.
28(1, S)
2014
Preliminary X-ray crystallographic analysis of glutathione transferase zeta 1 (GSTZ1a-1a).
Acta crystallographica. Section F, Structural biology communications.
70(Pt 2):187-9
[DOI] 10.1107/S2053230X13033591.
[PMID] 24637752.
2014
Seasonal influences on PCB retention and biotransformation in fish.
Environmental science and pollution research international.
21(10):6324-33
[DOI] 10.1007/s11356-013-1611-3.
[PMID] 23494683.
2014
Triclosan and Estradiol Sulfate Induce Similar Genomic Responses in the Late Gestation Ovine Fetal Hypothalamus
Reproductive Sciences.
21(3, S)
2013
Interactions of cytosolic sulfotransferases with xenobiotics.
Drug metabolism reviews.
45(4):401-14
[DOI] 10.3109/03602532.2013.835613.
[PMID] 24188364.
2013
Isoform-Selective Glucuronidation of Triclosan
The FASEB's Journal.
27
2013
Pharmacokinetics of oral dichloroacetate in dogs.
Journal of biochemical and molecular toxicology.
27(12):522-5
[DOI] 10.1002/jbt.21518.
[PMID] 24038869.
2012
Prenatal and postnatal expression of glutathione transferase ζ 1 in human liver and the roles of haplotype and subject age in determining activity with dichloroacetate.
Drug metabolism and disposition: the biological fate of chemicals.
40(2):232-9
[DOI] 10.1124/dmd.111.041533.
[PMID] 22028318.
2012
Slow O-demethylation of methyl triclosan to triclosan, which is rapidly glucuronidated and sulfonated in channel catfish liver and intestine.
Aquatic toxicology (Amsterdam, Netherlands).
124-125:72-82
[DOI] 10.1016/j.aquatox.2012.07.009.
[PMID] 22926334.
2012
Triclosan Sulfonation By Human Cytosolic Sulfotransferases
Drug Metabolism Reviews.
44
2011
Biological effects of Byrsocarpus coccineus in vitro.
Pharmaceutical biology.
49(2):152-60
[DOI] 10.3109/13880209.2010.504967.
[PMID] 21110733.
2011
Dichloroacetate-Dependent Inactivation of Gstz1 in Human Liver Cytosol Is Modulated By Physiologically Important Anions in a Gstz1 Haplotype-Dependent Manner
Drug Metabolism Reviews.
43
2011
Mitochondrion as a novel site of dichloroacetate biotransformation by glutathione transferase zeta 1.
The Journal of pharmacology and experimental therapeutics.
336(1):87-94
[DOI] 10.1124/jpet.110.173195.
[PMID] 20884751.
2011
Ontogeny of Hepatic Glutathione Transferase Enzymes in Humans
Drug Metabolism Reviews.
43
2011
Steroid catabolism in marine and freshwater fish.
The Journal of steroid biochemistry and molecular biology.
127(3-5):167-75
[DOI] 10.1016/j.jsbmb.2010.10.003.
[PMID] 20955793.
2010
Triclosan is a potent inhibitor of estradiol and estrone sulfonation in sheep placenta.
Environment international.
36(8):942-9
[DOI] 10.1016/j.envint.2009.02.004.
[PMID] 19299018.
2009
Influence of dietary Coexposure to benzo(a)pyrene on the biotransformation and distribution of 14C-methoxychlor in the channel catfish (Ictalurus punctatus).
Toxicological sciences : an official journal of the Society of Toxicology.
108(2):320-9
[DOI] 10.1093/toxsci/kfp018.
[PMID] 19181613.
2009
The state of in vitro science for use in bioaccumulation assessments for fish.
Environmental toxicology and chemistry.
28(1):86-96
[DOI] 10.1897/08-015.1.
[PMID] 18717614.
2008
Age-dependent kinetics and metabolism of dichloroacetate: possible relevance to toxicity.
The Journal of pharmacology and experimental therapeutics.
324(3):1163-71
[PMID] 18096758.
2008
Effects of Food Natural Products on the Biotransformation of PCBs.
Environmental toxicology and pharmacology.
25(2):211-7
[DOI] 10.1016/j.etap.2007.10.024.
[PMID] 19255595.
2008
Effects of the pesticide methoxychlor on gene expression in the liver and testes of the male largemouth bass
.
(86):459-469
2008
Effects of the pesticide methoxychlor on gene expression in the liver and testes of the male largemouth bass
.
(86):459-469
2008
Effects of the pesticide methoxychlor on gene expression in the liver and testes of the male largemouth bass (Micropterus salmoides).
Aquatic toxicology (Amsterdam, Netherlands).
86(4):459-69
[DOI] 10.1016/j.aquatox.2007.12.008.
[PMID] 18279978.
2008
Glucuronidation and sulfonation, in vitro, of the major endocrine-active metabolites of methoxychlor in the channel catfish, Ictalurus punctatus, and induction following treatment with 3-methylcholanthrene.
Aquatic toxicology (Amsterdam, Netherlands).
86(2):227-38
[PMID] 18078677.
2008
Glucuronidation of polychlorinated biphenylols and UDP-glucuronic acid concentrations in channel catfish liver and intestine.
Drug metabolism and disposition: the biological fate of chemicals.
36(4):623-30
[DOI] 10.1124/dmd.107.019596.
[PMID] 18180271.
2008
Stimulation of transactivation of the largemouth bass estrogen receptors alpha, beta-a, and beta-b by methoxychlor and its mono- and bis-demethylated metabolites in HepG2 cells.
The Journal of steroid biochemistry and molecular biology.
108(1-2):55-63
[PMID] 17949972.
2008
Stimulation of transactivation of the largemouth bass estrogen receptors alpha, beta-α and beta-β by methoxychlor and its mono-and bis-demethylated metabolites in HepG2 cells
The Journal of steroid biochemistry and molecular biology.
(108):55-63
2008
Stimulation of transactivation of the largemouth bass estrogen receptors alpha, beta-α and beta-β by methoxychlor and its mono-and bis-demethylated metabolites in HepG2 cells
.
(108):55-63
2008
Tissue Distribution and Metabolism of C-14-Methoxichlor in Untreated and Benzo(A)Pyrene-Treated Channel Catfish
Marine Environmental Research.
66
2007
Sulfonation of 17beta-estradiol and inhibition of sulfotransferase activity by polychlorobiphenylols and celecoxib in channel catfish, Ictalurus punctatus.
Aquatic toxicology (Amsterdam, Netherlands).
81(3):286-92
[PMID] 17239972.
2006
Demethylation of the pesticide methoxychlor in liver and intestine from untreated, methoxychlor-treated, and 3-methylcholanthrene-treated channel catfish (Ictalurus punctatus): evidence for roles of CYP1 and CYP3A family isozymes.
Drug metabolism and disposition: the biological fate of chemicals.
34(6):932-8
[PMID] 16510540.
2006
Inhibition and recovery of rat hepatic glutathione S-transferase zeta and alteration of tyrosine metabolism following dichloroacetate exposure and withdrawal.
Drug metabolism and disposition: the biological fate of chemicals.
34(1):36-42
[PMID] 16199472.
2006
Inhibition of sulfotransferases by xenobiotics.
Current drug metabolism.
7(1):83-104
[PMID] 16454694.
2006
Intestinal bioavailability and biotransformation of 3,3′,4,4′-tetrachlorobiphenyl (CB 77) in in situ preparations of channel catfish following dietary induction of CYP1A.
Aquatic toxicology (Amsterdam, Netherlands).
77(1):33-42
[PMID] 16364462.
2006
Polychlorobiphenylols are selective inhibitors of human phenol sulfotransferase 1A1 with 4-nitrophenol as a substrate.
Chemico-biological interactions.
159(3):235-46
[PMID] 16413005.
2005
In vitro inhibition of human hepatic and cDNA-expressed sulfotransferase activity with 3-hydroxybenzo[a]pyrene by polychlorobiphenylols.
Environmental health perspectives.
113(6):680-7
[PMID] 15929889.
2005
Properties and regional expression of a CYP3A-like protein in channel catfish intestine.
Aquatic toxicology (Amsterdam, Netherlands).
72(4):361-71
[PMID] 15848255.
2005
Sulfonation of environmental chemicals and their metabolites in the polar bear (Ursus maritimus).
Drug metabolism and disposition: the biological fate of chemicals.
33(9):1341-8
[PMID] 15951448.
2005
Sulfotransferase 2A1 forms estradiol-17-sulfate and celecoxib switches the dominant product from estradiol-3-sulfate to estradiol-17-sulfate.
The Journal of steroid biochemistry and molecular biology.
96(5):367-74
[PMID] 16011896.
2004
Glucuronidation in the polar bear (Ursus maritimus).
Marine environmental research.
58(2-5):475-9
[PMID] 15178068.
2004
Increased toxicity of benzo(a)pyrene-7,8-dihydrodiol in the presence of polychlorobiphenylols.
Marine environmental research.
58(2-5):343-6
[PMID] 15178052.
2004
Liquid chromatography-tandem mass spectrometry method for the simultaneous determination of delta-ALA, tyrosine and creatinine in biological fluids.
Clinica chimica acta; international journal of clinical chemistry.
350(1-2):219-30
[PMID] 15530481.
2004
Triclosan as a substrate and inhibitor of 3′-phosphoadenosine 5′-phosphosulfate-sulfotransferase and UDP-glucuronosyl transferase in human liver fractions.
Drug metabolism and disposition: the biological fate of chemicals.
32(10):1162-9
[PMID] 15269185.
2004
Unified gas chromatographic-mass spectrometric method for quantitating tyrosine metabolites in urine and plasma.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.
808(2):153-61
[PMID] 15261808.
2003
Binding of 3-hydroxybenzo[a]pyrene to bovine hemoglobin and albumin.
Journal of biochemical and molecular toxicology.
17(4):239-47
[PMID] 12898648.
2003
Pharmacologic or genetic ablation of maleylacetoacetate isomerase increases levels of toxic tyrosine catabolites in rodents.
Biochemical pharmacology.
66(10):2029-38
[PMID] 14599561.
2002
Hydroxylated polychlorinated biphenyls as inhibitors of the sulfation and glucuronidation of 3-hydroxy-benzo[a]pyrene.
Environmental health perspectives.
110(4):343-8
[PMID] 11940451.
2002
Intestinal and hepatic microsomal metabolism of testosterone and progesterone by a 3 alpha-hydroxysteroid dehydrogenase to the 3 alpha-hydroxy derivatives in the channel catfish, Ictalurus punctatus.
The Journal of steroid biochemistry and molecular biology.
82(4-5):413-24
[PMID] 12589949.
2001
Intestinal bioavailability and biotransformation of 3-hydroxybenzo(a)pyrene in an isolated perfused preparation from channel catfish, Ictalurus punctatus.
Drug metabolism and disposition: the biological fate of chemicals.
29(5):721-8
[PMID] 11302939.
2000
Differential expression of alpha-like glutathione S-transferase (GST) isoforms in catfish intestine.
Marine environmental research.
50(1-5):353-6
[PMID] 11460717.
2000
Effect of micelle fatty acid composition and 3,4,3′, 4′-tetrachlorobiphenyl (TCB) exposure on intestinal [(14)C]-TCB bioavailability and biotransformation in channel catfish in situ preparations.
Toxicological sciences : an official journal of the Society of Toxicology.
55(1):85-96
[PMID] 10788563.
2000
Oral bioavailability and pharmacokinetics of elimination of 9-hydroxybenzo[a]pyrene and its glucoside and sulfate conjugates after administration to male and female American lobsters, Homarus americanus.
Toxicological sciences : an official journal of the Society of Toxicology.
57(1):75-86
[PMID] 10966513.
2000
Purification and characterization of hepatic and intestinal phenol sulfotransferase with high affinity for benzo[a]pyrene phenols from channel catfish, Ictalurus punctatus.
Archives of biochemistry and biophysics.
376(2):409-19
[PMID] 10775429.
2000
Sulfation and glucuronidation of benzo[a]pyrene-7,8-dihydrodiol in intestinal mucosa of channel catfish (Ictalurus punctatus).
Marine environmental research.
50(1-5):11-5
[PMID] 11460675.
2000
The influence of diet on the regional distribution of glutathione S-transferase activity in channel catfish intestine.
Journal of biochemical and molecular toxicology.
14(3):148-54
[PMID] 10711630.
1999
Determination of chloral hydrate metabolites in human plasma by gas chromatography-mass spectrometry.
Journal of pharmaceutical and biomedical analysis.
19(3-4):309-18
[PMID] 10704096.
1999
Inhibition of glutathione S-transferase zeta and tyrosine metabolism by dichloroacetate: a potential unifying mechanism for its altered biotransformation and toxicity.
Biochemical and biophysical research communications.
262(3):752-6
[PMID] 10471397.
1998
Bioavailability and biotransformation of benzo(a)pyrene in an isolated perfused In situ catfish intestinal preparation.
Environmental health perspectives.
106(3):155-66
[PMID] 9449680.
1998
Clinical pharmacology and toxicology of dichloroacetate.
Environmental health perspectives.
106 Suppl 4(Suppl 4):989-94
[PMID] 9703483.
1998
Cytochromes P450 in crustacea.
Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology.
121(1-3):157-72
[PMID] 9972457.
1998
Pharmacokinetics and metabolism of [14C]dichloroacetate in male Sprague-Dawley rats. Identification of glycine conjugates, including hippurate, as urinary metabolites of dichloroacetate.
Drug metabolism and disposition: the biological fate of chemicals.
26(11):1134-43
[PMID] 9806957.
1998
Pharmacokinetics, metabolism and toxicology of dichloroacetate.
Drug metabolism reviews.
30(3):499-539
[PMID] 9710704.
1997
Determination of dichloroacetate and its metabolites in human plasma by gas chromatography-mass spectrometry.
Journal of chromatography. B, Biomedical sciences and applications.
703(1-2):75-84
[PMID] 9448064.
1997
Dietary modulation of phase 1 and phase 2 activities with benzo(a)pyrene and related compounds in the intestine but not the liver of the channel catfish, Ictalurus punctatus.
Drug metabolism and disposition: the biological fate of chemicals.
25(3):346-54
[PMID] 9172953.
1997
Glutathione-dependent conversion to glyoxylate, a major pathway of dichloroacetate biotransformation in hepatic cytosol from humans and rats, is reduced in dichloroacetate-treated rats.
Drug metabolism and disposition: the biological fate of chemicals.
25(11):1223-7
[PMID] 9351896.
1997
Kinetics and metabolism of chloral hydrate in children: identification of dichloroacetate as a metabolite.
Biochemical and biophysical research communications.
235(3):695-8
[PMID] 9207222.
1997
Pharmacokinetics of 2-naphthol following intrapericardial administration, and formation of 2-naphthyl-beta-D-glucoside and 2-naphthyl sulphate in the American lobster, Homarus americanus.
Xenobiotica; the fate of foreign compounds in biological systems.
27(6):609-26
[PMID] 9211660.
1996
cDNA and protein sequence of a major form of P450, CYP2L, in the hepatopancreas of the spiny lobster, Panulirus argus.
Archives of biochemistry and biophysics.
329(1):31-8
[PMID] 8619632.
1995
Biotransformation, hepatopancreas DNA binding and pharmacokinetics of benzo[a]pyrene after oral and parenteral administration to the American lobster, Homarus americanus.
Chemico-biological interactions.
95(1-2):141-60
[PMID] 7697746.
1994
Oral bioavailability and disposition of sulphadimethoxine in lobster, Homarus americanus, following single and multiple dosing regimens.
Xenobiotica; the fate of foreign compounds in biological systems.
24(9):921-31
[PMID] 7810173.
1992
Kinetic and inhibitor studies of acetaminophen and zidovudine glucuronidation in rat liver microsomes.
Drug and chemical toxicology.
15(2):161-75
[PMID] 1597129.
1991
Rapid, dose-dependent elimination of phenol across the gills, and slow elimination of phenyl sulfate in the urine of phenol-dosed lobsters, Homarus americanus.
Drug metabolism and disposition: the biological fate of chemicals.
19(2):536-42
[PMID] 1676666.
1991
Southern flounder hepatic and intestinal metabolism and DNA binding of benzo[a]pyrene (BaP) metabolites following dietary administration of low doses of BaP, BaP-7,8-dihydrodiol or a BaP metabolite mixture.
Chemico-biological interactions.
79(3):305-21
[PMID] 1913975.
1990
Bioavailability, metabolism, and renal excretion of benzoic acid in the channel catfish (Ictalurus punctatus).
Drug metabolism and disposition: the biological fate of chemicals.
18(5):552-6
[PMID] 1981701.
1990
Isolation of cytochrome P450 from hepatopancreas microsomes of the spiny lobster, Panulirus argus, and determination of catalytic activity with NADPH cytochrome P450 reductase from vertebrate liver.
Archives of biochemistry and biophysics.
282(1):8-17
[PMID] 2171437.
1989
Cytochrome P450 monooxygenases in crustaceans.
Xenobiotica; the fate of foreign compounds in biological systems.
19(10):1063-76
[PMID] 2683409.
1989
Glucose and sulfate conjugation of phenolic compounds by the spiny lobster (Panulirus argus).
Journal of biochemical toxicology.
4(2):133-8
[PMID] 2512388.
1988
Acute and subacute effects of miconazole nitrate on hepatic styrene oxide hydrolase and cytochrome P-450-dependent monooxygenase activities in male and female AKR/J mice.
Toxicology.
50(3):269-81
[PMID] 3394155.
1987
Conjugation of organic pollutants in aquatic species.
Environmental health perspectives.
71:97-103
[PMID] 3297669.
1987
Formation of benzo[a]pyrene-DNA adducts by microsomal enzymes: comparison of maternal and fetal liver, fetal hematopoietic cells and placenta.
Chemico-biological interactions.
61(3):203-14
[PMID] 3568191.
1987
In vivo and in vitro renal metabolism and excretion of benzoic acid by a marine teleost, the southern flounder.
Drug metabolism and disposition: the biological fate of chemicals.
15(5):665-70
[PMID] 2891484.
1986
1-14C-n-hexadecane disposition in the spiny lobster, Panulirus argus and the American lobster, Homarus americanus.
Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer.
6(5-6):13-27
[PMID] 3783435.
1986
Determination of ketoconazole in the plasma, liver, lung and adrenal of the rat by high-performance liquid chromatography.
Journal of chromatography.
377:287-94
[PMID] 3711219.
1986
Differential induction of ethoxyresorufin-O-deethylase in tissues of the rat placenta.
The Journal of pharmacology and experimental therapeutics.
238(3):1108-13
[PMID] 3746661.
1986
Induction of cytochrome P-450c in hematopoietic cells of fetal liver.
Biochemical and biophysical research communications.
141(1):299-305
[PMID] 3541928.
1986
Overview of in vitro metabolism of drugs by aquatic species.
Veterinary and human toxicology.
28 Suppl 1:2-8
[PMID] 3151420.
1986
Positional metabolism of benzo(a)pyrene in rat placenta and maternal liver. Comparison of induction effects.
Drug metabolism and disposition: the biological fate of chemicals.
14(4):471-6
[PMID] 2873995.
1985
Structural features of imidazole derivatives that enhance styrene oxide hydrolase activity in rat hepatic microsomes.
Journal of medicinal chemistry.
28(8):1120-4
[PMID] 4020834.
1985
Temperature-dependent disposition of [14C]benzo(a)pyrene in the spiny lobster, Panulirus argus.
Toxicology and applied pharmacology.
77(2):325-33
[PMID] 3975903.
1984
3-Methylcholanthrene does not induce in vitro xenobiotic metabolism in spiny lobster hepatopancreas, or affect in vivo disposition of benzo[a]pyrene.
Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology.
78(1):241-5
[PMID] 6146477.
1984
Benzo(a)pyrene metabolism in hepatic microsomes from feral and 3-methycholanthrene-treated southern flounder, Paralichthys lethostigma.
Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer.
5(4-5):309-20
[PMID] 6520733.
1984
Catalytic properties of cytochrome P-450 in hepatopancreas of the spiny lobster, Panulirus argus
Marine Environmental Research.
14(1-4):1-11
[DOI] 10.1016/0141-1136(84)90066-7.
1984
Cytochrome P-450-dependent oxidation of progesterone, testosterone, and ecdysone in the spiny lobster, Panulirus argus.
Archives of biochemistry and biophysics.
233(1):1-9
[PMID] 6431908.
1983
Modification of benzo(a)pyrene metabolism in hepatic microsomes from untreated and induced rats by imidazole derivatives which inhibit monooxygenase activity and enhance epoxide hydrolase activity.
Drug metabolism and disposition: the biological fate of chemicals.
11(4):350-4
[PMID] 6137342.
1982
Disposition and taurine conjugation of 2,4-dichlorophenoxyacetic acid, 2,4,5-trichlorophenoxyacetic acid, bis(4-chlorophenyl)acetic acid, and phenylacetic acid in the spiny lobster, Panulirus argus.
Drug metabolism and disposition: the biological fate of chemicals.
10(5):516-22
[PMID] 6128203.
1981
Imidazole derivatives as inhibitors of cytochrome P-450-dependent oxidation and activators of epoxide hydrolase in hepatic microsomes from a marine fish.
Biochemical pharmacology.
30(20):2876-80
[PMID] 6274351.
1981
Polyhalogenated biphenyls and phenobarbital: evaluation as inducers of drug metabolizing enzymes in the sheepshead, Archosargus probatocephalus.
Chemico-biological interactions.
36(2):229-48
[PMID] 6268313.
1980
Polycyclic aromatic hydrocarbon induction of cytochrome P-450-dependent mixed-function oxidases in marine fish.
Toxicology and applied pharmacology.
54(1):117-33
[PMID] 7394780.
1979
Epoxide hydrase and glutathione S-transferase activities with selected alkene and adrene oxides in several marine species.
Chemico-biological interactions.
25(2-3):321-44
[PMID] 466737.
1979
Hepatic microsomal mixed-function oxidase activities in several marine species common to coastal Florida
Comparative Biochemistry and Physiology Part C: Comparative Pharmacology.
62(2):155-164
[DOI] 10.1016/0306-4492(79)90004-2.
1978
A radiochemical assay for glycine N-acyltransferase activity. Some properties of the enzyme in rat and rabbit.
The Biochemical journal.
172(2):285-91
[PMID] 666745.
1978
In vitro metabolism of xenobiotics in some marine animals.
Annals of the New York Academy of Sciences.
298:505-21
[PMID] 360912.
1978
Perinatal development of, and effect of chemical pretreatment on, glycine N-acyltransferase activities in liver and kidney of rabbit and rat.
The Biochemical journal.
172(2):293-9
[PMID] 666746.
1977
Fate and distribution of the herbicides 2,4-dichlor-phenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) in the dogfish shark.
Xenobiotica; the fate of foreign compounds in biological systems.
7(10):623-31
[PMID] 910463.
1977
The perinatal development of epoxide-metabolizing enzyme activities in liver and extrahepatic organs of guinea pig and rabbit.
Drug metabolism and disposition: the biological fate of chemicals.
5(1):19-28
[PMID] 13972.
1976
Characteristics and development of drug metabolism by pulmonary microsomes.
Agents and actions.
6(4):527-30
[PMID] 785983.
1976
Hepatic and extra hepatic metabolism, in vitro, of an epoxide (8-14C-styrene oxide) in the rabbit
Biochemical Pharmacology.
25(2):187-193
[PMID] 4076.
1976
Hepatic and extrahepatic metabolism of 14C-styrene oxide.
Environmental health perspectives.
17:135-44
[PMID] 1026399.
1976
Taurine conjugation of 2,4-dichlorophenoxyacetic acid and phenylacetic acid in two marine species.
Xenobiotica; the fate of foreign compounds in biological systems.
6(7):393-8
[PMID] 997587.
1973
The conjugation of phenylacetic acid in phenylketonurics.
European Journal of Clinical Pharmacology.
5:243-246
[DOI] 10.1007/BF00567012.
1972
The Conjugation of 4-Chloro- and 4-Nitro-phenylacetic Acids in Man, Monkey and Rat
Xenobiotica.
2(5):499-506
[DOI] 10.3109/00498257209111078.
[PMID] 4630830.
1972
The conjugation of phenylacetic acid in man, sub-human primates and some non-primate species
Proceedings of the Royal Society of London. Series B. Biological Sciences.
182(1066):25-35
[DOI] 10.1098/rspb.1972.0064.
1972
The rate of procaine hydrolysis in serum of normal subjects and diseased patients.
Clinical Pharmacology and Therapeutics.
13:279-284
Grants
Mar 2019
– Aug 2019
Molecular and Genomic Approaches in Alleviating Environmental Pollution
Role: Principal Investigator
Funding: EGYPTIAN CULTURAL AND EDU BUR
via US AGENCY FOR INTERNATIONAL DEVELOPMENT
Sep 2016
– Aug 2021
Developmental Pharmacology of Mitochondrial and Cytosolic GSTZ1
Role: Principal Investigator
Funding: NATL INST OF HLTH NIGMS
Sep 2012
– Aug 2016
Developmental Pharmacology of Cytosolic and Mitochondrials GSTZ1-1/MAAI
Role: Principal Investigator
Funding: NATL INST OF HLTH NIGMS
Jun 2011
– Apr 2017
Health Impact of Deepwater Horizon Spill in Eastern Gulf Coast Communities
Role: Project Manager
Funding: NATL INST OF HLTH NIEHS
Education
D.Sc.
1993
·
University of London
Post-doctoral Fellow
1972-1975
·
National Institute of Environmental Health Sciences
Ph.D.
1972
·
St. Mary’s Medical School
B.Sc.
1969
·
University College London
Contact Details
Phones:
- Business:
- (352) 273-7714
Emails:
- Business:
- mojames@ufl.edu
Addresses:
- Business Mailing:
-
PO Box 100485
GAINESVILLE FL 32610 - Business Street:
-
MSB P6-29
1345 Center Drive
GAINESVILLE FL 326103003